Alcohol Dehydrogenase (ADH) Reaction
Exercise 1: Alcohol Dehydrogenase (ADH) Reaction
Bich 413 section 508
Abstract
The Alcohol Dehydrogenase Reaction in Oxidized Nicotinamide Adenine Dinucleotide.
The oxidation-reduction of Nicotinamide adenine Dinucleotides (NADH) are very Important reaction in the biological system. When the Oxidized Nicotinamide Adenine Dinucleotide (NAD+) is react with a alcohol, catalyze by Alcohol Dehydrogenase (ADH), the result products are NADH, H+, and aldehyde.In the biological system, the ADH reaction can go both forward and reverse reaction depended on the concentration of NAD+, NADH, and Alcohol. In the TCA cycle, NADH is the main product. We are studying how the concentration and types of alcohol can affect the ADH reaction. A spectrophotometer is used to determine the concentration of NADH produced in the reaction. Since NADH shows a maximum absorbance at 340 nm that is absent in NAD+, the concentration of NADH can be determine by the measurement of the absorbance. It was found that when high concentration of alcohol was used, the concentration of NADH will be higher. However the equilibrium constants remain the same in different concentration of alcohol. The type of alcohol was found to have an affect of the rate of the reaction. Ethanol has the higher reaction rate than n-propanol, n-butanol, and iso-butanol.
Exercise 1: Alcohol Dehydrogenase
4. Table for calculation of [NADH]eq, [NAD+]eq, [Ethanol]eq Keq={NADH] [Acetaldehyde] / [NAD+] [Ethanol] c=A/ ab a=6.2 mM-1cm-1
[NADH]eq [Acetaldehyde]eq [NAD+]eq [NAD+] [Ethanol] [Ethanol] Keq (mM) (mM) (mM) (mM) (mM) (mM)
1 Blank -- -- -- -- 0 --
2 0.012 0.012 0.088 0.1 19.99 20 8.19x10-5
3 0.022 0.022 0.078 0.1 59.98 60 10.3x10-5
4 0.028 0.028 0.072
Bich 413 section 508
Abstract
The Alcohol Dehydrogenase Reaction in Oxidized Nicotinamide Adenine Dinucleotide.
The oxidation-reduction of Nicotinamide adenine Dinucleotides (NADH) are very Important reaction in the biological system. When the Oxidized Nicotinamide Adenine Dinucleotide (NAD+) is react with a alcohol, catalyze by Alcohol Dehydrogenase (ADH), the result products are NADH, H+, and aldehyde.In the biological system, the ADH reaction can go both forward and reverse reaction depended on the concentration of NAD+, NADH, and Alcohol. In the TCA cycle, NADH is the main product. We are studying how the concentration and types of alcohol can affect the ADH reaction. A spectrophotometer is used to determine the concentration of NADH produced in the reaction. Since NADH shows a maximum absorbance at 340 nm that is absent in NAD+, the concentration of NADH can be determine by the measurement of the absorbance. It was found that when high concentration of alcohol was used, the concentration of NADH will be higher. However the equilibrium constants remain the same in different concentration of alcohol. The type of alcohol was found to have an affect of the rate of the reaction. Ethanol has the higher reaction rate than n-propanol, n-butanol, and iso-butanol.
Exercise 1: Alcohol Dehydrogenase
4. Table for calculation of [NADH]eq, [NAD+]eq, [Ethanol]eq Keq={NADH] [Acetaldehyde] / [NAD+] [Ethanol] c=A/ ab a=6.2 mM-1cm-1
[NADH]eq [Acetaldehyde]eq [NAD+]eq [NAD+] [Ethanol] [Ethanol] Keq (mM) (mM) (mM) (mM) (mM) (mM)
1 Blank -- -- -- -- 0 --
2 0.012 0.012 0.088 0.1 19.99 20 8.19x10-5
3 0.022 0.022 0.078 0.1 59.98 60 10.3x10-5
4 0.028 0.028 0.072