The beaker containing the two goldfish in 200 milliliters of fish water were transferred to the oxygenated chamber. Then another 200 milliliters of fish water was measured in a beaker and poured into the oxygenated chamber, creating a total of 400 milliliters of fish water in the chamber. The oxygenated chamber was then secured with the lid. The lid was pressed down until a small amount of fish water sprouted out to the top. This step is necessary because when the fish are in a small, closed system, it is easier to calculate oxygen consumption of the fish. After leaving the fish in the chamber for two minutes, The LoggerLite program was run for 30 seconds to ensure that the fish were accustomed to their new environment. The data for these 30 seconds was then …show more content…
After this transferring, the beaker contained caffeinated water which could not be placed in the fish tank. In order to remove the fish from the caffeinated water, the beaker with the two fish and the caffeinated water was dumped into a bucket. Using a fishnet, the caffeinated water was dumped into the sink from the bucket and the fishnet was left with the two fish. The two fish were then quickly transferred into the fish tank. Replace with (the two fish were taken out of the chamber, placed into a beaker, and drained from the caffeinated water before being placed back in the fish tank.) Before beginning the second trial, the chamber was emptied and carefully rinsed with distilled water. The second trial used this same procedure except with two new fishes. Their weight was also measured to make sure the size of the fish did not affect the data of oxygen consumption. The control group and the experimental groups were led the same way as trial one with the control group in pure fish water and experimental group using the rest of the 100 milliliters caffeinated water left from trial