25-30 Gcse For The Synthesis Of Chalcone

Chalcones were synthesized according to the previously described methodology (26). A substituted acetophenone (5 mmol) and benzaldehyde (5 mmol) were dissolved in dry methanol (30 ml) and catalytic amount of sodium hydroxide was added to the above solution. The reaction mixture was stirred for 30 min and then neutralized with 1 N HCl. The products were purified by recrystallization from ethyl alcohol and water. The synthetic procedure and reaction conditions for chalcones are illustrated in scheme 1
Animals
Adult male Swiss albino mice (25-30 g) were used for this study with access to food and water ad libitum. The animals were housed at 24 ± 2°C and relative humidity 55 ± 5 with 12:12 h light and dark cycle. The animals were acclimatizated
All other chemicals were of analytical grade. Mice were randomly divided into groups, each containing six animals. On the day of the experiment, control group received 0.1% carboxy-methyl cellulose, p.o., 1 h before the experiment. Chalcones (50 mg/kg) were suspended in 0.1% CMC and administered orally to animals 1 h before the test. The following two analgesic models were used to evaluate the synthesized
The amount of time (latency) in seconds, for withdrawal from water was recorded as reaction time. A cut off (maximum duration) of 15 sec was set to avoid tissue damage (28).
Statistical Analysis
All data were expressed as mean ± SEM. Statistical analysis was carried out by one-way ANOVA followed by Dennett’s test. The values considered significant at P<0.05 when compared with the control group.
RESULTS AND DISCUSSION
Chalcones containing different electron withdrawing and electron releasing substituent groups on various positions of ring B (3a-j) were subjected to molecular docking studies. Based on the results observed in these studies, we have synthesized the chalcones and screened them for their potential central antinociceptive activity using pain models such as tail immersion assay and hot-plate tests.
Molecular Docking