AP Biology Enzyme Lab Write-Up
AP Biology
Sunday, October 24, 2010 Enzyme Lab Write-Up
Purpose: To observe how an enzyme affect the speed of chemical reaction. To describe how the concentration of an enzyme affects its ability to work.
Hypothesis: Depending on the concentration of the catalase which the disk is soaked in, it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas.
Prediction: Since the rate of reaction can be lowered by the addition of catalysis such as an enzyme. Moreover to increase the effectiveness of an enzyme one must add additional amount of enzyme due to saturation. Therefore the 100% will have the greatest effect because is has the highest concentration of the catalase. …show more content…
Independent variable: The independent variable is the Potato extract.
Dependent variable: The dependent variable is hydrogen peroxide.
Controlled variable: Amount of hydrogen peroxide Potato extract Size of paper disk Temperature of potato extract
Level of treatment: Our level of treatment for the independent variable is based in 20 intervals. 20%, 40%, 60%, 80%, 100%
Materials: 2000 ml of potato extract 100 ml graduated cylinder 3% H2O2 solution Marking pen Knife Filter paper disk Stopwatch 1000 ml of H20 8 - 100 ml beakers Forceps Hot plate Cheesecloth
Procedure:
Label six beakers in intervals of 20%
Obtain catalase solution from your teacher and dilute according to chart on lab
Label another 100 ml beaker 3% H2O2.
Label the 50 ml ‘reaction chamber’
Using forceps, carefully dip one disk into a beaker
Hold in liquid for about three-seconds and then touch the disk to the inside of the beaker to remove excess drops of liquid.
Place the filer paper disk on the bottom of the beaker which contains hydrogen peroxide and release it saying now to your timekeeper.
Record how long it takes for the disk to rise to the surface.
Questions:
The catalysis solution is stored on ice so it will not react.
40%
We remove the extra catlike so that there is no extra solution on the disk and so that the results are not skewed.
It is important to use only one disk because the enzyme would take longer to react through two disk, and it would take more time for it to rise to the top of the solution. Oxygen gas was released by the reaction.
The arrows in this reaction represent that energy is being released, also imply’s that the reaction is
exothermic.
The concentration is in direct relation as the rate of reaction so as the concentration decreases so does the rate of reaction.
The reaction time changes because as the concentrations differ, there is less substance for the substance to react to.
Class data because you can take a class average. More trials. The catlike solution looked as if it turned greenish/grayish. Most likely it will react better with the H2O2. Enzymes function better in a higher temperature. N/A
The disks in the water had no signs of reacting. Optimum refers to the best temperature and pH the enzyme will function at.
Reaction 1: 7 Reaction 2: 7 Reaction 3: 110 Reaction 4: 80 Reaction 5: 60
Peroxisomes are organelles that are digestive, they help break down long chains of fatty acids. Also can be used to breakdown toxins that enter the cell. These functions are especially key in the lier and kidneys.
Conclusion: The main idea’s that I have taken away from this lab that enzymes perform better under optimal conditions. Also that the rate of reaction is directly related to the concentration of the substance.
Sunday, October 24, 2010 Enzyme Lab Write-Up
Purpose: To observe how an enzyme affect the speed of chemical reaction. To describe how the concentration of an enzyme affects its ability to work.
Hypothesis: Depending on the concentration of the catalase which the disk is soaked in, it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas.
Prediction: Since the rate of reaction can be lowered by the addition of catalysis such as an enzyme. Moreover to increase the effectiveness of an enzyme one must add additional amount of enzyme due to saturation. Therefore the 100% will have the greatest effect because is has the highest concentration of the catalase. …show more content…
Independent variable: The independent variable is the Potato extract.
Dependent variable: The dependent variable is hydrogen peroxide.
Controlled variable: Amount of hydrogen peroxide Potato extract Size of paper disk Temperature of potato extract
Level of treatment: Our level of treatment for the independent variable is based in 20 intervals. 20%, 40%, 60%, 80%, 100%
Materials: 2000 ml of potato extract 100 ml graduated cylinder 3% H2O2 solution Marking pen Knife Filter paper disk Stopwatch 1000 ml of H20 8 - 100 ml beakers Forceps Hot plate Cheesecloth
Procedure:
Label six beakers in intervals of 20%
Obtain catalase solution from your teacher and dilute according to chart on lab
Label another 100 ml beaker 3% H2O2.
Label the 50 ml ‘reaction chamber’
Using forceps, carefully dip one disk into a beaker
Hold in liquid for about three-seconds and then touch the disk to the inside of the beaker to remove excess drops of liquid.
Place the filer paper disk on the bottom of the beaker which contains hydrogen peroxide and release it saying now to your timekeeper.
Record how long it takes for the disk to rise to the surface.
Questions:
The catalysis solution is stored on ice so it will not react.
40%
We remove the extra catlike so that there is no extra solution on the disk and so that the results are not skewed.
It is important to use only one disk because the enzyme would take longer to react through two disk, and it would take more time for it to rise to the top of the solution. Oxygen gas was released by the reaction.
The arrows in this reaction represent that energy is being released, also imply’s that the reaction is
exothermic.
The concentration is in direct relation as the rate of reaction so as the concentration decreases so does the rate of reaction.
The reaction time changes because as the concentrations differ, there is less substance for the substance to react to.
Class data because you can take a class average. More trials. The catlike solution looked as if it turned greenish/grayish. Most likely it will react better with the H2O2. Enzymes function better in a higher temperature. N/A
The disks in the water had no signs of reacting. Optimum refers to the best temperature and pH the enzyme will function at.
Reaction 1: 7 Reaction 2: 7 Reaction 3: 110 Reaction 4: 80 Reaction 5: 60
Peroxisomes are organelles that are digestive, they help break down long chains of fatty acids. Also can be used to breakdown toxins that enter the cell. These functions are especially key in the lier and kidneys.
Conclusion: The main idea’s that I have taken away from this lab that enzymes perform better under optimal conditions. Also that the rate of reaction is directly related to the concentration of the substance.