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Absorbance Vs. Concentration Brapch

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Absorbance Vs. Concentration Brapch
Abstract: During the lab a better agent for absorbing Procion Red Dye was trying to be found. Using the different dilutions of a stock solution and a spectrophotometer an Absorbance vs. Concentration graph was created and using the trendline from that graph it was determined which agent, zeolite, magnetic zeolite, or charcoal were better at absorbing the dye. From the results collected it was found that charcoal was the best at absorbing the dye, when moles of dye per gram of agent used were calculated.
Introduction: Polycyclic Aromatic Hydrocarbons, PAHs, are commonly found in the environment due to industrial and agricultural processes and can be harmful if there are high concentrations in the environment because they can be very toxic 1.
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Concentration (mol/L) the trendline represents A=Ɛbc. A represents the y- values, the different absorbance values, Ɛb represents the slope, Ɛ=13255 M-1·cm-1 and b =1 cm, and finally c represents the x-values, the different concentrations. Therefore the trendline tells us that the molar absorptivity is equivalent to 13255 M-1·cm-1. Also the trendline is used to calculate the different concentrations of the zeolite, magnetized zeolite, and charcoal by plugging in the corrected absorbance of each of these agents into the y value of the equation and solving for x, which again represents …show more content…
Using the trendline equation y=13255x, you plug 0.009 into the y value, 0.009= 13255x, to get a concentration of 6.79x10-7 M. Change of Concentration=Initial Concentration- Calculated Concentration, 5.0x10-5M -6.79x10-7M= 4.93x10-5M. Then you calculate moles by the equation Moles= Concentration x Volume of Red Dye, 4.93x10-5 M x 0.0100 L= 4.93x10-7 mol. Then finally you divide moles of red dye grams of agent used, moles of dye per gram = 4.93x10-7 mol /0.1021gram =4.83x10-6 mols/gram. These calculations will also be done for the zeolite and magnetized zeolite to see which agent works the best. All the calculated values are found in Data Table 3: Charcoal, Zeolite, and Magnetized Zeolite Effectiveness and it can be seen that charcoal has the largest value of moles of dye absorbed per gram of agent used, therefore it is the best

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