Acacia Catechu Willh Case Study
9. Summary and Conclusion
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9.1 Herbal drugs are economical and their sources are plenty in India. As many synthetic show adverse effects. Attention of the healthcare practioners is directed towards herbal formulations to treat diseases with safety, effectiveness and stability. Majority of Indian population have the access to practice traditional or herbal medicines. Acacia catechu Willd. (family: Fabaceae) known as Black Cutch is used in traditional medical system for various diseases. Various parts of the tree viz, bark, leaves and heartwood possesses diverse pharmacological actions. Many studies have been carried out to evaluate its antibacterial, antioxidant, and antiinflammatory and antidiabetic activity.
As no Pharmacological …show more content…
Seeds. Phytochemical analysis were performed as per Trease and Evans method. Heavy metal analysis, presence of pesticides, aflatoxin in Acacia catechu seeds was investigated. Atomic absorption spectroscopy were followed for detection of heavy metals, Gc-MS analysis were performed for the detection of pesticide residues. HPTLC fingerprinting method was adopted for the detection of aflatoxin in Acacia catechu seeds. From the studies it was observed that the heavy metals present in Acacia catechu seeds were within the permissible limits. The presence of pesticides was detected as per standard protocol. The results showed the absence of pesticides in the Acacia catechu seeds. The HPTLC profile also confirmed the absence of aflatoxin in Acacia catechu seed extract. The extract also possessed significant antioxidant …show more content…
A. catechu leaves, heartwood and bark showed significant anticancer effects when tested In vitro. Hence work has been carried out in Acacia catechu seeds to explore the anticancer effects. Acacia catechu seeds were investigated against HepG2 (Human hepatocellular carcinoma) cell lines. Treatment exhibited potent cytotoxic activity in a dose dependent manner with an IC50 of 77. 0 µg/mL. The cytotoxic activity was associated with an increase in ROS(reactive oxygen species) generation and decrease in the activity of Super oxide dismutase and reduced glutathion content. Dual staining with ethidium bromide and propidium Iodide staining revealed that HepG2 cells treated with A.catechu seed extract caused cell death mainly by apoptosis. The events of cytotoxicity and oxidative stress were found to be associated with up-regulation of pro-apoptotic gene expressions such as bax, Cyt-c, caspase-3 and 9, and down-regulation of anti-apoptotic marker bcl-2. In addition, there was a significant increase of sub- G1 phase arrest in the cell cycle after A. catechu treatment. A. Catechu exerted its cytotoxic and antiproliferative effects by triggering apoptosis and cell cycle arrest in HepG2
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9.1 Herbal drugs are economical and their sources are plenty in India. As many synthetic show adverse effects. Attention of the healthcare practioners is directed towards herbal formulations to treat diseases with safety, effectiveness and stability. Majority of Indian population have the access to practice traditional or herbal medicines. Acacia catechu Willd. (family: Fabaceae) known as Black Cutch is used in traditional medical system for various diseases. Various parts of the tree viz, bark, leaves and heartwood possesses diverse pharmacological actions. Many studies have been carried out to evaluate its antibacterial, antioxidant, and antiinflammatory and antidiabetic activity.
As no Pharmacological …show more content…
Seeds. Phytochemical analysis were performed as per Trease and Evans method. Heavy metal analysis, presence of pesticides, aflatoxin in Acacia catechu seeds was investigated. Atomic absorption spectroscopy were followed for detection of heavy metals, Gc-MS analysis were performed for the detection of pesticide residues. HPTLC fingerprinting method was adopted for the detection of aflatoxin in Acacia catechu seeds. From the studies it was observed that the heavy metals present in Acacia catechu seeds were within the permissible limits. The presence of pesticides was detected as per standard protocol. The results showed the absence of pesticides in the Acacia catechu seeds. The HPTLC profile also confirmed the absence of aflatoxin in Acacia catechu seed extract. The extract also possessed significant antioxidant …show more content…
A. catechu leaves, heartwood and bark showed significant anticancer effects when tested In vitro. Hence work has been carried out in Acacia catechu seeds to explore the anticancer effects. Acacia catechu seeds were investigated against HepG2 (Human hepatocellular carcinoma) cell lines. Treatment exhibited potent cytotoxic activity in a dose dependent manner with an IC50 of 77. 0 µg/mL. The cytotoxic activity was associated with an increase in ROS(reactive oxygen species) generation and decrease in the activity of Super oxide dismutase and reduced glutathion content. Dual staining with ethidium bromide and propidium Iodide staining revealed that HepG2 cells treated with A.catechu seed extract caused cell death mainly by apoptosis. The events of cytotoxicity and oxidative stress were found to be associated with up-regulation of pro-apoptotic gene expressions such as bax, Cyt-c, caspase-3 and 9, and down-regulation of anti-apoptotic marker bcl-2. In addition, there was a significant increase of sub- G1 phase arrest in the cell cycle after A. catechu treatment. A. Catechu exerted its cytotoxic and antiproliferative effects by triggering apoptosis and cell cycle arrest in HepG2