Agrobacterium Tumefaciens Lab Report

II.Results and Discussion
A.Isolation of Agrobacterium tumefaciens:
Six bacterial colonies were observed and screened on the selective medium. The bacterial colonies was able to characterized after 72 hours of incubation, bacterial colonies were visible with naked eyes on YEMA plates. The colonies appeared were medium sized bubble shaped, round, regular, sticky and white coloured colonies were observed. From these initial results, the isolated bacteria were tentatively identified as Agrobacterium strains.
B.Characterization of Agrobacterium tumefaciens:
Biochemical tests: biochemical features of the selected isolates were given in the table 1. The Gram reaction indicates selected isolated are Gram negative. All the six isolated are positive
The negative control was E.Coli and positive control was identified Agarobacterium strain.

D.Molecular analysis on protein profiling of isolates:
The protein sample of six isolated was showed the distinguished protein bands in the range of 66KDa to 14KDa. The molecular weight sizing data showed that the all the six isolates were diverse Table 2. The data generated on the polymorphism study was confirmed the diversity in the isolates table 3.

Table 2: Data generated by Rf value of protein traveled and its Molecular weight by using molecular weight standard. Strain A1 Strain A2 Strain A3 Strain A4 Strain A5 Strain A6
No. Of bands Rf value Molecular weight (KDa) Rf value Molecular weight (KDa) Rf value Molecular weight (KDa) Rf value Molecular weight (KDa) Rf value Molecular weight (KDa) Rf value Molecular weight (KDa)
1 0.14 81 0.23 71 0.40 55 0.84 8.5 0.40 55 0.49 45.5
2 0.30 65 0.29 66 0.43 51 0.60 34.5 0.41 53.5 0.54 40
3 0.41 53.5 0.32 62 0.49 45.5 0.65 28 0.6
In previous studies effective protection against tetracyclin was obtained (18) and also confirms that the Agrobacterium tumefaciens have the sensitivity to many antibiotics including tetracyclin and kanamycin (19). Antibiotic resistance means the abililty of a microorganism to withstand the effects of an antibiotics(20). The isolated were showed sensitivity towards ampicillin, chloramphenicol, penicillin, streptomycin, sulphatriad confirms the differnce in response between the isolates should reflect natural