Alamar Blue Assay Lab Report

Alamar Blue assay are performed to show cell viability and proliferation. In the procedure we treated our cell line, SUM 149 cells with different concentrations of an unknown treatment. SUM 149 cells are an inflammatory breast cancer cell line. The treatments were used to suppress cell growth and proliferation. The station that I performed my work was station C. I platted my cells and aloud a previously determined allotted time for cell proliferation and growth to occur. The cells were then treated with varying concentrations of an unknown treatment to be administrated. Once the treatment was performed an Alamar Blue assay was then ran to assesse the effects of the treatment. The active ingredient in Alamar Blue is resazurin, which is reduced to resorufin that is red and fluorescent. The
The solutions 1-4 have readings closer to 0 than 1000. My control contained cells plus medium that were not treated has a reading approximately around 6,700. This contrast shows that the cells in the different concentrations of unknown treatment had low viability. The cells+medium control has high viability. A higher fluorescence reading means that there is a higher cell level. Solution two’s concentration was the most effective if the purpose of the unknown solutions is to hinder cell viability. The trend that the treatment solutions followed was dose dependent. This is due to the fact that as the concentration changed the viability changed the viability changed in a direct manner. This could not be considered threshold concentration due to the fact that the desired result was to limit cell viability and all of the cells that were treated showed a desired affect. Solution five is just another concentration and not a vehicle control because if it was then the vehicle control should have the same reading or close to the same reading as the cells+medium