Analysis Of Stevia Rebaudiana
Abstract:
Stevia rebaudiana is an herb with a very high medicinal value. It has more than 100 phytochemicals. It is used as sugar substitute. The extracts from leaves are used for the microbial assay and study the antimicrobial activity. The microbes used for the testing the activities are E.coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Aspergillusniger, Candida parapsilosis, Trichophyton rubrum. With different concentrations of the extracts starting from 2.5µg/ml, 5.0µg/ml, 7.5µg/ml, 1.0µg/ml and the highest activity is shown by Staphylococcus aureus and Candida parapsilosis.
Introduction:
We use natural products as therapeutic activity to protect ourselves from many microbes which has the ability to cause infection[1]. The natural products extracted from plant have a long history were the main source of medicines we use now a …show more content…
The fine round paper discs were obtained and were sterilized.
RESULTS AND DISCUSSION:
Antimicrobial activity:
The antimicrobial activity of leaf and root extracts were examined against Gram positive and Gram-negative bacteria and fungal strains by measuring zone of inhibition.
The antimicrobial activity was performed by Agar disc diffusion method at concentration level of 2.5, 5.0, 7.0, 10µg/ml respectively.
Ampicillin (antibacterial), Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in figures and tables.
Stevia rebaudiana leaves:
Organism/concn of extract 2.5µg/ml 5µg/ml 7.5µg/ml 10µg/ml
E.coli 1.0cm 1.1 cm 1.3 cm 1.5 cm
Staphylococcus aureus 1.1 cm 1.3cm 1.3 cm 1.5 cm
Bacillus subtilis 0.7 cm 0.9 cm 1.1 cm 1.2 cm
Salmonella typhi 0.8 cm 1.2 cm 1.3 cm 1.6
Stevia rebaudiana is an herb with a very high medicinal value. It has more than 100 phytochemicals. It is used as sugar substitute. The extracts from leaves are used for the microbial assay and study the antimicrobial activity. The microbes used for the testing the activities are E.coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Aspergillusniger, Candida parapsilosis, Trichophyton rubrum. With different concentrations of the extracts starting from 2.5µg/ml, 5.0µg/ml, 7.5µg/ml, 1.0µg/ml and the highest activity is shown by Staphylococcus aureus and Candida parapsilosis.
Introduction:
We use natural products as therapeutic activity to protect ourselves from many microbes which has the ability to cause infection[1]. The natural products extracted from plant have a long history were the main source of medicines we use now a …show more content…
The fine round paper discs were obtained and were sterilized.
RESULTS AND DISCUSSION:
Antimicrobial activity:
The antimicrobial activity of leaf and root extracts were examined against Gram positive and Gram-negative bacteria and fungal strains by measuring zone of inhibition.
The antimicrobial activity was performed by Agar disc diffusion method at concentration level of 2.5, 5.0, 7.0, 10µg/ml respectively.
Ampicillin (antibacterial), Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in figures and tables.
Stevia rebaudiana leaves:
Organism/concn of extract 2.5µg/ml 5µg/ml 7.5µg/ml 10µg/ml
E.coli 1.0cm 1.1 cm 1.3 cm 1.5 cm
Staphylococcus aureus 1.1 cm 1.3cm 1.3 cm 1.5 cm
Bacillus subtilis 0.7 cm 0.9 cm 1.1 cm 1.2 cm
Salmonella typhi 0.8 cm 1.2 cm 1.3 cm 1.6