First, Parmesan cheesemaking usually requires around 2 years in aging time in order to achieve desired characteristics (Wilkinson, 1993). The other one is the fact that rennet has not been commercialized yet in Indonesia. Imported rennet then drives the Parmesan cheesemaking manufacturing cost further up, driving potential investors away. Four major constituents in milk that play significant role in cheesemaking are casein, whey, lactose, and fat. Casein, accounting for 75-80% of total protein in milk, is present in form of micelles constituted of α-, β-, and κ-casein (Trejo, 2012). The presence of κ-casein stabilizes both α- and β-casein and prevents coagulation. However, κ-casein is inactivated upon rennet addition to the milk, allowing casein micelles to interact and form coagulates called “curd” while entrapping whey and fat globules inside. According to Fox, et al. (2000), an enzyme
First, Parmesan cheesemaking usually requires around 2 years in aging time in order to achieve desired characteristics (Wilkinson, 1993). The other one is the fact that rennet has not been commercialized yet in Indonesia. Imported rennet then drives the Parmesan cheesemaking manufacturing cost further up, driving potential investors away. Four major constituents in milk that play significant role in cheesemaking are casein, whey, lactose, and fat. Casein, accounting for 75-80% of total protein in milk, is present in form of micelles constituted of α-, β-, and κ-casein (Trejo, 2012). The presence of κ-casein stabilizes both α- and β-casein and prevents coagulation. However, κ-casein is inactivated upon rennet addition to the milk, allowing casein micelles to interact and form coagulates called “curd” while entrapping whey and fat globules inside. According to Fox, et al. (2000), an enzyme