Antibiotic Production
Antibiotic Production from the plant Lantana camara
Project Summary
The purpose of this experimental procedure is targeted to find a novel bacterium from the soil of Lantana camara that can be used in the dental field to kill or stop the growth of the Streptococcus mutans bacteria. The experimental procedure is to take place over a time frame of ten weeks conducted by a student team of four. The proposed methodology to take place is to cultivate and purify isolates from the soil, find antibiotic isolates, isolate the genomic DNA, to amplify the 16S rRNA gene segments by polymerase chain reaction, and to build a phylogenetic tree.
Lantana is a genus of about 150 species of flowering plants popularly used as antirheumatic, stimulant, antibacterial, biologic control and as ornamental plant (Dua VK et al., 1996). Lantana camara is a shrub that belongs to Verbenaceae family and is a native of America and Africa. Different parts of the plant, mainly the leaves, have been used in the treatment of swelling, stomachache, rheumatism, wound healing, biliary fever, toothache, bronquitis, antiseptic and other affections (Deena MJ et al., 2000).
In the literature “Chemical composition and resistance-modifying effect of the essential oil of Lantana camara Linn” the chemical constituents, antibacterial and modulatory activities of the essential oil of Lantana camara Linn were studied. The findings of the chemical study of essential oil of L. camara leaves revealed the presence of a high amount of sesquiterpenes and this oil remarkably inhibited the growth of most tested bacteria and fungi; Pseudomonas aeruginosa, Aspergillus niger, Fusarium solani and Candida albicans appearing as the most sensitive (Sousa et al., 2012). Considerable research, such as this one, has focused primarily on the antibacterial properties the essential oil of the Lantana camara has. This research being done is trying branch off of the studies on the essential oil of the plant and zone in on the
Project Summary
The purpose of this experimental procedure is targeted to find a novel bacterium from the soil of Lantana camara that can be used in the dental field to kill or stop the growth of the Streptococcus mutans bacteria. The experimental procedure is to take place over a time frame of ten weeks conducted by a student team of four. The proposed methodology to take place is to cultivate and purify isolates from the soil, find antibiotic isolates, isolate the genomic DNA, to amplify the 16S rRNA gene segments by polymerase chain reaction, and to build a phylogenetic tree.
Lantana is a genus of about 150 species of flowering plants popularly used as antirheumatic, stimulant, antibacterial, biologic control and as ornamental plant (Dua VK et al., 1996). Lantana camara is a shrub that belongs to Verbenaceae family and is a native of America and Africa. Different parts of the plant, mainly the leaves, have been used in the treatment of swelling, stomachache, rheumatism, wound healing, biliary fever, toothache, bronquitis, antiseptic and other affections (Deena MJ et al., 2000).
In the literature “Chemical composition and resistance-modifying effect of the essential oil of Lantana camara Linn” the chemical constituents, antibacterial and modulatory activities of the essential oil of Lantana camara Linn were studied. The findings of the chemical study of essential oil of L. camara leaves revealed the presence of a high amount of sesquiterpenes and this oil remarkably inhibited the growth of most tested bacteria and fungi; Pseudomonas aeruginosa, Aspergillus niger, Fusarium solani and Candida albicans appearing as the most sensitive (Sousa et al., 2012). Considerable research, such as this one, has focused primarily on the antibacterial properties the essential oil of the Lantana camara has. This research being done is trying branch off of the studies on the essential oil of the plant and zone in on the