Antimicrobial activity of soil against bacterial diarrhoea
(RESEARCH TREATISE)
By
SITUMBEKO LIWELEYA
(s213459531)
Submitted in fulfilment of the requirements for the degree of
BACHALOROUS TECHNOLOGIEA: BIOMEDICAL TECHNOLOGY
At the
At
Nelson Mandela Metropolitan University
Port Elizabeth, 2013.
SUPERVISOR- PROFESSOR SMITH. N.
DECLARATION
I, the undersigned, hereby declare that the research work contained in this study is my own original work, and all the sources I have used or quoted have been indicated and acknowledged by means of complete references.
Situmbeko Liweleya
ABSTRACT
Anecdotes, both historical and recent, recount the curing of skin infections, including diaper rash, by using red soils from the Hashemite Kingdom of Jordan. Following inoculation of red soils isolated from geographically separate areas of Jordan, Micrococcus luteus and Staphylococcus aureus were rapidly killed. Over the 3-week incubation period, the number of specific types of antibiotic-producing bacteria increased, and high antimicrobial activity (MIC, ∼10 μg/ml) was observed in methanol extracts of the inoculated red soils. Antibiotic-producing microorganisms whose numbers increased during incubation included actinomycetes, Lysobacter spp., and Bacillus spp. The actinomycetes produced actinomycin C2 and actinomycin C3. No myxobacteria or lytic bacteriophages with activity against either M. luteus or S. aureus were detected in either soil before or after inoculation and incubation. These results suggest that the antibiotic activity of Jordan's red soils is due to the proliferation of antibiotic-producing bacteria.
A total of 51 Actinomycetes were isolated from different soil samples of Palestine. Preliminary screening by cross-streak method was carried out for all the 51 isolates. After preliminary screening, 17 isolates which showed antimicrobial (antibacterial, antifungal) activity were selected for further study. Among these 17 isolates tested, 5 isolates
By
SITUMBEKO LIWELEYA
(s213459531)
Submitted in fulfilment of the requirements for the degree of
BACHALOROUS TECHNOLOGIEA: BIOMEDICAL TECHNOLOGY
At the
At
Nelson Mandela Metropolitan University
Port Elizabeth, 2013.
SUPERVISOR- PROFESSOR SMITH. N.
DECLARATION
I, the undersigned, hereby declare that the research work contained in this study is my own original work, and all the sources I have used or quoted have been indicated and acknowledged by means of complete references.
Situmbeko Liweleya
ABSTRACT
Anecdotes, both historical and recent, recount the curing of skin infections, including diaper rash, by using red soils from the Hashemite Kingdom of Jordan. Following inoculation of red soils isolated from geographically separate areas of Jordan, Micrococcus luteus and Staphylococcus aureus were rapidly killed. Over the 3-week incubation period, the number of specific types of antibiotic-producing bacteria increased, and high antimicrobial activity (MIC, ∼10 μg/ml) was observed in methanol extracts of the inoculated red soils. Antibiotic-producing microorganisms whose numbers increased during incubation included actinomycetes, Lysobacter spp., and Bacillus spp. The actinomycetes produced actinomycin C2 and actinomycin C3. No myxobacteria or lytic bacteriophages with activity against either M. luteus or S. aureus were detected in either soil before or after inoculation and incubation. These results suggest that the antibiotic activity of Jordan's red soils is due to the proliferation of antibiotic-producing bacteria.
A total of 51 Actinomycetes were isolated from different soil samples of Palestine. Preliminary screening by cross-streak method was carried out for all the 51 isolates. After preliminary screening, 17 isolates which showed antimicrobial (antibacterial, antifungal) activity were selected for further study. Among these 17 isolates tested, 5 isolates