Antimicrobial Activity of Soil- Research Proposal Abstract
ANTIMICROBIAL ACTIVITY OF SOIL BY SITUMBEKO LIWELEYA (s213459531) Research Proposal Submitted in fulfillment of the requirements for the degree of Bachelor of BIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | at Nelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGY, SUPERVISOR- PROFESSOR N. SMITH |
Contents ANTIMICROBIAL PROPERTIES OF SOIL 2 ABSTRACT 2 LITERATURE REVIEW 3 AIM OF RESEARCH 4 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4 METHOD(S) TO FACILITATE MICROBIAL IDENTIFICATION 5 Gram stain 5 Culturing 5 MATERIALS TO BE USED 5 ETHICAL CONSIDERATIONS 6 SAMPLE COLLECTION 6 SAMPLE ANALYSIS METHOD 7 DAY 3 – Expected Results 9 DISSEMINATION OF RESULTS 9 WORK AND TIME SCHEDULE 10 FINANCE BUDGET 10 CONCLUSION 12 REFERENCE LIST 13 BIBLIOGRAPHY 14
ANTIMICROBIAL PROPERTIES OF SOIL
ABSTRACT
Isolation and characterization of microorganisms is a practice that aids in increasing ones knowledge of a laboratory setting and it helps improve on using sterile technique. Microbes produce various antibiotics and by isolating different microbes the antibodies can be tested for. Soil samples are highly rich in microorganisms. The antimicrobial activities of microbes from 3 different soil sources (Tenby, Summerstrand, North Campus main building round-about and South Campus water pond) will be tested. Pure colonies isolated from the microbes will be tested for antimicrobial activity against known microorganisms of medical importance; these being E.coli, staphylococcus aureus, Shigella dysenteriae, Shigella sonnei and salmonella spp. Isolates of soil microbes can be categorized and characterized based on a number of criteria ranging from gram-staining which will be done in this project, for enumeration which is a quantitative description based on the amount of microbial colonies available. The results will be recorded.
Key words: soil, microbes, microbial activity, antimicrobial
Contents ANTIMICROBIAL PROPERTIES OF SOIL 2 ABSTRACT 2 LITERATURE REVIEW 3 AIM OF RESEARCH 4 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4 METHOD(S) TO FACILITATE MICROBIAL IDENTIFICATION 5 Gram stain 5 Culturing 5 MATERIALS TO BE USED 5 ETHICAL CONSIDERATIONS 6 SAMPLE COLLECTION 6 SAMPLE ANALYSIS METHOD 7 DAY 3 – Expected Results 9 DISSEMINATION OF RESULTS 9 WORK AND TIME SCHEDULE 10 FINANCE BUDGET 10 CONCLUSION 12 REFERENCE LIST 13 BIBLIOGRAPHY 14
ANTIMICROBIAL PROPERTIES OF SOIL
ABSTRACT
Isolation and characterization of microorganisms is a practice that aids in increasing ones knowledge of a laboratory setting and it helps improve on using sterile technique. Microbes produce various antibiotics and by isolating different microbes the antibodies can be tested for. Soil samples are highly rich in microorganisms. The antimicrobial activities of microbes from 3 different soil sources (Tenby, Summerstrand, North Campus main building round-about and South Campus water pond) will be tested. Pure colonies isolated from the microbes will be tested for antimicrobial activity against known microorganisms of medical importance; these being E.coli, staphylococcus aureus, Shigella dysenteriae, Shigella sonnei and salmonella spp. Isolates of soil microbes can be categorized and characterized based on a number of criteria ranging from gram-staining which will be done in this project, for enumeration which is a quantitative description based on the amount of microbial colonies available. The results will be recorded.
Key words: soil, microbes, microbial activity, antimicrobial
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