Antimicrobial Properties of Soil Used in the Treatment of Bacterial Diarrhoea
ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY, 2013.SUPERVISOR- PROFESSOR N. SMITH | |
Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA 3 ABSTRACT 3 LITERATURE REVIEW 3 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4 METHOD(S) 4 Wet preparation microscopy 4 Gram stain 4 Culturing 4 MATERIALS TO BE USED 5 Microorganisms known to cause diarrhoea 5 Other material 5 Wet preparation microscopy 5 Gram stain 6 Culture 6 Biochemical tests 6 Glass ware 7 ETHICAL CONSIDERATIONS 7 SAMPLING COLLECTION 7 SAMPLE ANALYSIS METHODS 8 EVALUATION OF RESULTS 8 DISSEMINATION OF RESEARCH RESULTS 9 WORK AND TIME SCHEDULE 9 FINANCE BUDGET 9 Other requirements 9 Wet preparation microscopy 9 Gram stain 10 Culture 10 Biochemical tests 10 CONCLUSION 11 REFERENCE LIST 12
ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
ABSTRACT
Bacilli class of domain bacteria, mostly found in soil form a tough protective endospore, allowing these organisms to tolerate extreme environmental conditions. The crude metabolite extracted from Bacilli class of soil microorganisms, particularly B. methylotrophicus-SCS2012 exhibited strong antibacterial activity against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae with the zone of inhibition range 28.33 to 32.16 mm at 100μg/disc. Minimum inhibitory concentration (MIC) of the extract against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae was found to be 156, 156, 312, 312 and 625μg/ml respectively.
Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA 3 ABSTRACT 3 LITERATURE REVIEW 3 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4 METHOD(S) 4 Wet preparation microscopy 4 Gram stain 4 Culturing 4 MATERIALS TO BE USED 5 Microorganisms known to cause diarrhoea 5 Other material 5 Wet preparation microscopy 5 Gram stain 6 Culture 6 Biochemical tests 6 Glass ware 7 ETHICAL CONSIDERATIONS 7 SAMPLING COLLECTION 7 SAMPLE ANALYSIS METHODS 8 EVALUATION OF RESULTS 8 DISSEMINATION OF RESEARCH RESULTS 9 WORK AND TIME SCHEDULE 9 FINANCE BUDGET 9 Other requirements 9 Wet preparation microscopy 9 Gram stain 10 Culture 10 Biochemical tests 10 CONCLUSION 11 REFERENCE LIST 12
ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
ABSTRACT
Bacilli class of domain bacteria, mostly found in soil form a tough protective endospore, allowing these organisms to tolerate extreme environmental conditions. The crude metabolite extracted from Bacilli class of soil microorganisms, particularly B. methylotrophicus-SCS2012 exhibited strong antibacterial activity against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae with the zone of inhibition range 28.33 to 32.16 mm at 100μg/disc. Minimum inhibitory concentration (MIC) of the extract against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae was found to be 156, 156, 312, 312 and 625μg/ml respectively.