ONPG Yellow coloured suspension formed +
ADH Yellow coloured suspension formed -
LDC Orange coloured suspension formed with precipitate +
ODC Yellow coloured suspension formed -
CIT Greenish suspension formed -
H2S The suspension remain colourless -
URE Yellow coloured suspension formed -
TDA Yellow coloured suspension formed -
IND Red coloured suspension formed with precipitate +
VP The colourless suspension become pinkish +
GEL The suspension remain colourless -
GLU The colourless suspension turn yellow in colour +
MAN Yellow coloured suspension formed +
INO Blue coloured suspension formed -
SOR Yellow coloured suspension formed +
RHA Yellow coloured suspension formed +xidase negative Enterobacteriaceae or otherwise the test will be non- reliable. An oxidase negative bacteria refers to bacteria that do not use oxygen as its electron receptor in their electron transport chain.
All of the microtubes contain ingredients that act as indicator for their reactions except for the TDA, IND and VP
SAC Blue coloured suspension formed -
MEL Blue coloured suspension formed -
AMY Blue coloured suspension formed -
ARA Yellow coloured suspension formed +
• Result of API 20 E refers to the presence of Escherichia coli 1 and Escherichia 2 in the bacterial suspension.
Analytical profile index which is also known as API is generally used for rapid identification of classified microorganisms. This API 20E kit contained 20 microtubes that contain dehydrated substrates. In the experiment, we supposed to fill all of the 20 microtubes with the bacterial suspension. However, due to a misunderstanding, we made a mistake where we do not fill the OPNG, TDA, IND, GLU, MAN, INO, SOR, RHA, SAC, MEL, AMY, ARA, OX microtubes with the bacterial suspension. The result shown above was obtained from our colleagues with 85.3 % identification of Escherichia Coli. After the inoculation of the API strip, the inoculated strip was then incubated in its chamber for 18 to 24 hours at 37 ºC. When the incubation period was done, an oxidase test was performed first to ensure that it is an oxidase negative Enterobacteriaceae or otherwise the test will be non- reliable. An oxidase negative bacteria refers to bacteria that do not use oxygen as its electron receptor in their electron transport chain.
All of the microtubes contain ingredients that act as indicator for their reactions except for the TDA, IND and VP tubes which do not have indicator within the substrates. Most of the tubes will react by changing the colour due to pH changes and certain tubes changes colour with the help of the reagent in order to detect the metabolic product and reactions such as fermentation or oxidation for example for the tube containing inositol,
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