Arabidopsis Research Paper

Overview: The long term of this project is to know the price that resisting plants paid to be resistance against pathogens. Arabidopsis is the nominated plant for this research because it is the model plant that can be controlled. Also, it is the first plant that has the resistance gene that we are focusing on. This approach will be applied to other plants that have this resistance gene like tomato. Thus, the outcome of the resistance plants would be quantitatively measured and decided before applying it on the field. That would be economically highly effective to reduce the loss of the plant and evaluate productivity.
Intellectual Merit: This research depend upon one of the resistance genes that had been found in Arabidopsis, which is SRFR1
To fight off infection, the plant has an innate immune system and that works to overcome pathogens. Intercellular immune response induces resistance proteins that are activated by the pathogen effector proteins that injected inside cells by pathogen. This effectors protein is recognized by effector-triggered immunity (ETI) (Chisholm et al., 2006; Jones and Dangl, 2006). The immune system needs efficient regulation to resist pathogen. One of this regulator is called SRFR1 (suppressor of rps4-RLD 1) that is a negative regulator that provides a balance between immune system repression and activation (Kwon et al., 2009). SRFR1 is suppressor of RPS-RLD. RPS4 is a resistance gene in Arabidopsis to Pseudomonas syringae (DC3000) bacteria that express AvrRps4. Resistance only ensues when both genes are present that are the resistance protein and the avrRps4 avirulence protein (Kwon et al.,
The srfr1-1, srfr1-2 were the first mutations from SRFR1 gene. These mutations are recessive and located on two independent genes in the bottom of the fourth chromosome of Arabidopsis. The difference between these two mutations is that both are not allelic mutant srfr1-1 and srfr1-2. Also, they are located in two separate complementation groups. They do not work as an additive resistance, but they are fully susceptible to virulent DC3000. The resistance of both of them are specific to Pseudomonas syringae pv. tomato strain DC3000 expressing avrRps4(kwon et al., 2004). Actually, they have a separate pathway. They activate a gene-for-gene resistance response, but that is not functional on RLD (Kwon et al.,