Aseptic Technique Lab
Aseptic Technique and Culturing Microbes
Lab 3
Brittney Case
August 4th 2013
Section: Mic 101 Microbiology Session 1
Abstract:
The Purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures, learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly.
Hypothesis:
This exercise will allow me to gain knowledge of how to properly use aseptic techniques, become familiar with basic requirements of microbial growth, cultured media and methods to control their growth. This will further my knowledge and skills in making observations about specimens.
Methodology:
Materials:
-Small cardboard box or Styrofoam Cooler -Microscope
-Immersion Oil -Desk Lamp
-Aluminum Foil -Paper Towels
-10% bleach solution -Gloves
-S. epidermidis sample -Goggles
-Thermometer -Apron
-Test-tube rack -Candles
-Slide box with blank slides -Cover slides
-Broth MRS-9ml -Broth, Nutrient
-Lactobacillus acidophilus -Sterile swab
-Gram stain solution #1 crystal violet -Mask
-Inoculation loop
Procedure:
-Set up incubation site
-Determine medium type
-Generate microbial cultures
-Observe my microbial cultures
* for a more detailed outline of procedures and step by step process please refer to the Labpaq manual.
Observation and Results:
Photo is of my constructed incubator without the tin foil. I have opted to place the incubator on top of my fridge, hoping that this would lead to less disturbance and curiosity from others in the household and was a validly suggested place for incubation in the lab manual.
Observations after 24 hours incubation:
Temperature at approx.: 37 degrees C
Nutrient Broth – S. epidermis: showing no signs of growth
MRS Broth – L. acidophilus: significant amount of growth
Observations after 48 hours incubation:
Temperature at approx.: 37
Lab 3
Brittney Case
August 4th 2013
Section: Mic 101 Microbiology Session 1
Abstract:
The Purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures, learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly.
Hypothesis:
This exercise will allow me to gain knowledge of how to properly use aseptic techniques, become familiar with basic requirements of microbial growth, cultured media and methods to control their growth. This will further my knowledge and skills in making observations about specimens.
Methodology:
Materials:
-Small cardboard box or Styrofoam Cooler -Microscope
-Immersion Oil -Desk Lamp
-Aluminum Foil -Paper Towels
-10% bleach solution -Gloves
-S. epidermidis sample -Goggles
-Thermometer -Apron
-Test-tube rack -Candles
-Slide box with blank slides -Cover slides
-Broth MRS-9ml -Broth, Nutrient
-Lactobacillus acidophilus -Sterile swab
-Gram stain solution #1 crystal violet -Mask
-Inoculation loop
Procedure:
-Set up incubation site
-Determine medium type
-Generate microbial cultures
-Observe my microbial cultures
* for a more detailed outline of procedures and step by step process please refer to the Labpaq manual.
Observation and Results:
Photo is of my constructed incubator without the tin foil. I have opted to place the incubator on top of my fridge, hoping that this would lead to less disturbance and curiosity from others in the household and was a validly suggested place for incubation in the lab manual.
Observations after 24 hours incubation:
Temperature at approx.: 37 degrees C
Nutrient Broth – S. epidermis: showing no signs of growth
MRS Broth – L. acidophilus: significant amount of growth
Observations after 48 hours incubation:
Temperature at approx.: 37
References: http://www.cdc.gov/hipac/disinfection_sterilization/13_11sterilizingpractices.html Http:/www.britannica.com/EBchecked/topic/483854/pure-culture www.thefreedictionary.com