ASSIGNMENT 3 BIOMOLECS
Ethanol Emulsion Test: Assignment 3
Introduction:
The purpose of this lab was to test for the presence of lipids. Lipids consist of fats and oils. The test performed is called the Ethanol Emulsion test. The solubility of lipids and ethanol were broken down in this test. Lipids are non-polar organic compounds, making them un-soluble in water but soluble in organic solvents, such as ethanol. Sudan IV solution tested the oil in each sample by dying the lipids red and rising the oil to the top of the solution. The hypothesis was that the whole and soy milk were going to test negative for the presence of lipids. The null hypothesis was that eggs, lemon-lime soda, and Coca-Cola all tested negative for the presence of lipids, not only whole and skim milk.
Methods:
The emulsion test began with making a control group, a negative test and a positive test. The negative test was made with just 1 mL of distilled water and 4 mL of ethanol. The positive test was made with 1 drop of butter and 4 mL of ethanol. Then both test tubes were shaken to split the ester linkages and hydrolyze the lipids. Both negative and positive tests tubes had 20 drops of Sudan IV added into them so the presence of lipids could be shown. In the butter test tube (the positive control group) the oil was dyed red and rose to the top of the test tube. In the test tube with distilled water (the negative control group) no changes occurred. Afterwards various food samples were crushed and mixed with ethanol to test for the presence of lipids. Then each test tube was shaken, and 20 drops of Sudan IV solution was added. All food sample changes that occurred were recorded in a table.
Results:
After both control groups were made, the rest of the food samples were tested. About 1 mL of Olive Oil was mixed in a test tube with 4 mL of ethanol then shaken so that when the 20 drops of Sudan IV was added, the oil would dye red and rise to the top of the test tube. Afterwards vegetable oil and lemon juice were tested
Introduction:
The purpose of this lab was to test for the presence of lipids. Lipids consist of fats and oils. The test performed is called the Ethanol Emulsion test. The solubility of lipids and ethanol were broken down in this test. Lipids are non-polar organic compounds, making them un-soluble in water but soluble in organic solvents, such as ethanol. Sudan IV solution tested the oil in each sample by dying the lipids red and rising the oil to the top of the solution. The hypothesis was that the whole and soy milk were going to test negative for the presence of lipids. The null hypothesis was that eggs, lemon-lime soda, and Coca-Cola all tested negative for the presence of lipids, not only whole and skim milk.
Methods:
The emulsion test began with making a control group, a negative test and a positive test. The negative test was made with just 1 mL of distilled water and 4 mL of ethanol. The positive test was made with 1 drop of butter and 4 mL of ethanol. Then both test tubes were shaken to split the ester linkages and hydrolyze the lipids. Both negative and positive tests tubes had 20 drops of Sudan IV added into them so the presence of lipids could be shown. In the butter test tube (the positive control group) the oil was dyed red and rose to the top of the test tube. In the test tube with distilled water (the negative control group) no changes occurred. Afterwards various food samples were crushed and mixed with ethanol to test for the presence of lipids. Then each test tube was shaken, and 20 drops of Sudan IV solution was added. All food sample changes that occurred were recorded in a table.
Results:
After both control groups were made, the rest of the food samples were tested. About 1 mL of Olive Oil was mixed in a test tube with 4 mL of ethanol then shaken so that when the 20 drops of Sudan IV was added, the oil would dye red and rise to the top of the test tube. Afterwards vegetable oil and lemon juice were tested