Asystasia Gangetica Case Study
Previously, Yeoh, & Wong (1993) reported the nutritional values of A. gangetica. Ogle et al., 2001 reported the folate composition of A. gangetica. However, not much information on the utility of this leafy vegetable has been reported. To the best of our knowledge, there is no report on the optimization of polyphenols and their radical scavenging activities from the edible leaves of A. gangetica. Therefore, the main aim of this study is to optimize extraction conditions such as EtOH concentration, liquid-to-solid ratio and extraction time to obtain the maximum extraction yield, phenolic content, flavonoid content and DPPH and ABTS radical scavenging activity from the A. gangetica leaves. Furthermore, the polyphenols present in the optimized
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Anderson leaves (AGL) were collected in the month of October, 2015, from spontaneous plants in Sri Adhivaraganallur, Cuddalore District, Tamil Nadu, located in the Southeast part of India. The collected samples were identified by Southern Regional Centre, Botanical Survey of India (BSI), TNAU Campus, Coimbatore, India (voucher specimen no. BSI/SRC/5/23/2013-14/Tech/1922) and the voucher specimen was deposited in the university department. The fresh leaves were washed with tape and distilled water, shade dried for one week and then ground to a powdered in domestic mixer. The grounded powder sample was passed through a 60-mesh sieve (to accomplish uniform particle size) and stored in an airtight container for future …show more content…
For extraction, 1g of AGL powder was transferred to a screw capped glass tube and placed in an ultrasonic bath according to the experimental design presented in the Table 2 with varying EtOH concentration (X1: 40–80 %), liquid-to-solid ratio (X2: 10-–30 mL/g) and extraction time (X3: 30–50 min) with fixed ultrasonic frequency (40 kHz) and power (110 W) at constant room temperature (Wong Paz et al., 2015). After extraction, the extracts were filtered (Whatman no.1 filter paper) and centrifuged at 6000 rpm for 20 min at 40C (5430R Centrifuge, Eppendorf, Belgium). Again the supernatant was filtered (0.45 µm syringe filter) and stored at −800C in ultra-low temperature Panasonic MDF-U55V-PE deep freezer (Sci-Med (Asia) Pte. Ltd., Singapore) until analysis. All the experiments were done in
Anderson leaves (AGL) were collected in the month of October, 2015, from spontaneous plants in Sri Adhivaraganallur, Cuddalore District, Tamil Nadu, located in the Southeast part of India. The collected samples were identified by Southern Regional Centre, Botanical Survey of India (BSI), TNAU Campus, Coimbatore, India (voucher specimen no. BSI/SRC/5/23/2013-14/Tech/1922) and the voucher specimen was deposited in the university department. The fresh leaves were washed with tape and distilled water, shade dried for one week and then ground to a powdered in domestic mixer. The grounded powder sample was passed through a 60-mesh sieve (to accomplish uniform particle size) and stored in an airtight container for future …show more content…
For extraction, 1g of AGL powder was transferred to a screw capped glass tube and placed in an ultrasonic bath according to the experimental design presented in the Table 2 with varying EtOH concentration (X1: 40–80 %), liquid-to-solid ratio (X2: 10-–30 mL/g) and extraction time (X3: 30–50 min) with fixed ultrasonic frequency (40 kHz) and power (110 W) at constant room temperature (Wong Paz et al., 2015). After extraction, the extracts were filtered (Whatman no.1 filter paper) and centrifuged at 6000 rpm for 20 min at 40C (5430R Centrifuge, Eppendorf, Belgium). Again the supernatant was filtered (0.45 µm syringe filter) and stored at −800C in ultra-low temperature Panasonic MDF-U55V-PE deep freezer (Sci-Med (Asia) Pte. Ltd., Singapore) until analysis. All the experiments were done in