Bacteria and Motility Medium Tubes
Questions
A. Define the following terms:
1. Monotrichous
2. Amphitrichous
3. Lophotrichous
4. Peritrichous
B. What are the three commonly used techniques to test motility?
C. Why are semi-solid media used to test for motility?
D. Why might it be difficult to observe motility in a wet mount?
E. Why is it important to use a needle rather than an inoculating loop when inoculating a motility tube? F. For which of the organisms on the wet mount, if any, were you able to observe motility?
G. For which of the organisms in the motility medium tubes, if any, were you able to observe motility?
Questions
A. Define the following terms:
1. Monotrichous
2. Amphitrichous
3. Lophotrichous
4. Peritrichous
B. What are the three commonly used techniques to test motility?
C. Why are semi-solid media used to test for motility?
D. Why might it be difficult to observe motility in a wet mount?
E. Why is it important to use a needle rather than an inoculating loop when inoculating a motility tube? F. For which of the organisms on the wet mount, if any, were you able to observe motility?
G. For which of the organisms in the motility medium tubes, if any, were you able to observe motility?
Carbohydrate Fermentation Testing
Questions
A. What is fermentation?
B. Why is it important not to incubate the fermentation tubes beyond 24 hours?
C. Why is phenol red added to the fermentation tubes?
D. Why do bacteria have differences in the carbohydrates they can ferment?
E. Why does the formation of yellow color indicate fermentation?
F. What information can be gained by running a fermentation series on a particular microbe?
G. What does a dark pink or red color indicate?
H. What is the source of the air bubble that may form in the Durham tube?
A. Define the following terms:
1. Monotrichous
2. Amphitrichous
3. Lophotrichous
4. Peritrichous
B. What are the three commonly used techniques to test motility?
C. Why are semi-solid media used to test for motility?
D. Why might it be difficult to observe motility in a wet mount?
E. Why is it important to use a needle rather than an inoculating loop when inoculating a motility tube? F. For which of the organisms on the wet mount, if any, were you able to observe motility?
G. For which of the organisms in the motility medium tubes, if any, were you able to observe motility?
Questions
A. Define the following terms:
1. Monotrichous
2. Amphitrichous
3. Lophotrichous
4. Peritrichous
B. What are the three commonly used techniques to test motility?
C. Why are semi-solid media used to test for motility?
D. Why might it be difficult to observe motility in a wet mount?
E. Why is it important to use a needle rather than an inoculating loop when inoculating a motility tube? F. For which of the organisms on the wet mount, if any, were you able to observe motility?
G. For which of the organisms in the motility medium tubes, if any, were you able to observe motility?
Carbohydrate Fermentation Testing
Questions
A. What is fermentation?
B. Why is it important not to incubate the fermentation tubes beyond 24 hours?
C. Why is phenol red added to the fermentation tubes?
D. Why do bacteria have differences in the carbohydrates they can ferment?
E. Why does the formation of yellow color indicate fermentation?
F. What information can be gained by running a fermentation series on a particular microbe?
G. What does a dark pink or red color indicate?
H. What is the source of the air bubble that may form in the Durham tube?