Bacteria Classification by Gram Staining
Bacteria Classification By Gram Staining
THE AMERICAN UNIVERSITY IN CAIRO
BIOLOGY DEPARTMENT
SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1
Presented By : Karim A. Zaklama 92-1509 Sci. 453-01
24/2/96
Objective:
To test a sample of laboratory prepared bacteria and categorise it according to Christian's gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics.
Introduction:
Bacteria was categorised into two groups in 1884 by the Danish
Bacteriologist Christian, gram positive and gram negative by a staining technique where the ability to avoid de-coloration of Crystal Violet solution by alcohol would render the category of gram positive, and gram negative if the bacteria is de-coloured. This could be noted by the final colour of the bacteria: a violet colour where Gram positive and a pink colour of the Safranin added pending the de-colouring process.
Materials:
1. Bacteria Sample 2. Microscope Slide 3. Gram Staining Kit and Wash Bottles
a. Crystal Violet Solution b. Iodine Solution c. 95% Ethyl Alcohol d.
Safranin e. Distilled Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil
Procedure:
A. Preparation :
1. Bacteria is cultivated on agar jelly in an incubator at 25°C for 24 hours. 2. Obtain a microscope slide and with a toothpick, smear a thin coat of the bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet and leave standing for 20 seconds 4. Wash off the stain with distilled water; drain and blot off the excess with bibulous paper. 5. Apply Gram's Iodine on the smear and leave to stand for 1 minute. 6. Drain the excess iodine and apply
95% Ethyl alcohol for 20 second duration or till the alcohol runs clearly from the slide. 7. The smear should rinsed for a few seconds with distilled water to stop the action of the alcohol. 8. Drain and blot off the excess with bibulous
9. Introduce Safranin to
THE AMERICAN UNIVERSITY IN CAIRO
BIOLOGY DEPARTMENT
SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1
Presented By : Karim A. Zaklama 92-1509 Sci. 453-01
24/2/96
Objective:
To test a sample of laboratory prepared bacteria and categorise it according to Christian's gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics.
Introduction:
Bacteria was categorised into two groups in 1884 by the Danish
Bacteriologist Christian, gram positive and gram negative by a staining technique where the ability to avoid de-coloration of Crystal Violet solution by alcohol would render the category of gram positive, and gram negative if the bacteria is de-coloured. This could be noted by the final colour of the bacteria: a violet colour where Gram positive and a pink colour of the Safranin added pending the de-colouring process.
Materials:
1. Bacteria Sample 2. Microscope Slide 3. Gram Staining Kit and Wash Bottles
a. Crystal Violet Solution b. Iodine Solution c. 95% Ethyl Alcohol d.
Safranin e. Distilled Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil
Procedure:
A. Preparation :
1. Bacteria is cultivated on agar jelly in an incubator at 25°C for 24 hours. 2. Obtain a microscope slide and with a toothpick, smear a thin coat of the bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet and leave standing for 20 seconds 4. Wash off the stain with distilled water; drain and blot off the excess with bibulous paper. 5. Apply Gram's Iodine on the smear and leave to stand for 1 minute. 6. Drain the excess iodine and apply
95% Ethyl alcohol for 20 second duration or till the alcohol runs clearly from the slide. 7. The smear should rinsed for a few seconds with distilled water to stop the action of the alcohol. 8. Drain and blot off the excess with bibulous
9. Introduce Safranin to