In my test the s. epidermidis turned bright red indicating a positive result meaning that it produces a stable acid. The E.coli sample turned orange…
When unknown 32 was tested with the oxidase test, it was found oxidase negative. The catalase test tests for the presence of catalase which breaks down hydrogen peroxide and oxidase dismutase. A small amount of hydrogen peroxide is dropped onto a slide of unknown 32 and resulted in bubbles. This result shows that unknown 32 is catalase positive since it broke down hydrogen peroxide and oxidase dismutase which resulted in water and oxygen. This eliminated Enterococcus spp.…
When you did your hand wash experiment, you decided a variable to test, say, hot vs cold water, you called this variable an “experimental variable” since it was the variable you were testing experimentally. In order to get meaningful data, you need to keep other things the same, for example the washing time had to be the same for both, you called the washing time a _Control_______ variable.…
The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus aureus with a positive confirmatory test, mannitol salt agar, showing consistent results as well for S. aureus. The Gram-negative bacteria were Pseudomonas aeruginosa with a positive confirmatory…
From the streak plate, several slides were made to determine the morphology of unknown 45. A Gram stain, used to indicate the type of cell wall possessed by the bacterium, was performed. U45 stained a bright pink color and were small ovals and rods in shape. This indicated that U45 was a gram-negative bacillus bacterium. A capsular stain was then performed to determine the presence or absence of a capsule. Unknown 45 was positive for capsules. Having determined that U45 was gram-negative, there was no need to perform the Schaeffer-Fulton endospore stain, which is used detect the presence of endospores in certain gram-positive bacteria.…
In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is a fermenter they will use the sugars to make ATP. If the bacteria is a fermenter of lactose/sucrose the EMB plate we used will “clearly differentiate between the colonies of lactose fermenting and non-fermenting microbes. In the same medium sucrose was also included to differentiate between coliforms that were able to ferment sucrose more rapidly than those that were unable to ferment sucrose” (Cheeptham & Lal, 2007).…
This test is performed to get a description of the make-up of the cell membrane of the bacteria. A gram positive bacteria will display a blue/violet pigmentation where a gram negative bacteria will display a pink/red pigmentation. This is due to the outer membrane of gram negative bacteria. My unknown bacteria displays a pink color hinting the presence of a gram negative bacteria.…
The tests performed on the unknown bacteria cultures were all used to determine the identity of the bacteria. Each of the tests performed provided some key information about the bacteria in question and how it functions. Not all of the tests were performed on every culture, however, as some of the tests were used only for gram (+) or gram () bacteria, while others were even more specific and used only for cocci bacteria. The tests performed and what constitutes a positive and negative test are as follows.…
On the plate the bacteria produces enzymes called hemolysins these enzymes lyse the red blood cells to certain degrees. Bacteria the fully lyse the blood go through beta-hemolysis. Bacteria that partially lyse the blood go through alpha-hemolysis, and bacteria that doesn’t lyse at all goes through gamma hemolysis.…
An unknown bacterium experiment, this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al., 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by means of the Embden-Meyerhof pathway, they produce plasmid-encoded proteins and that some of them perform mixed acid fermentation, some use enzyme systems like formate dehydrogenase and pyruvate formate-lyase (PFL) The first thing that had to be done was the streak plating to isolate the bacterium, then the next lab multiple tests were ran to help identify which bacterium was in the sample. One of the most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium.…
The lactose test appeared pink still, which indicated no fermentation had happen and no gas was found either. The sucrose test appeared yellow indicating that sucrose was fermented turning the tube acidic and surprisingly no gas was found. After these tests and with the red growth on the TSA media it was confirmed that it was indeed the bacteria Serratia marcescens. In the gram-positive bacteria tests the MSA media turned completely yellow, which indicated mannitol was fermented turning the plate acidic. The glucose-bromocresol purple tube appeared completely yellow as well indicating glucose was fermented which turned the agar acidic. The bacteria Staphylococcus aureus was identified because it's the only gram-positive bacteria, which is able to turn both MSA and glucose-bromocrosel tests acidic, which gives them their yellow appearance. No further testing was needed to identify the two…
The change from negative serum, without antibodies specific to an infecting agent, to positive serum, containing antibodies against that infecting agent, is called…
This was done using an inoculating needle and aseptically transferring the bacteria into a slant of Simmon's citrate agar by stabbing the needle into the butt of the agar, then streaking it across the top of the agar as the needle was pulled out. The tube was then placed in the 37 degrees Celsius incubator for 48 hours, observed for a blue color, then placed back in the incubator for another 5 days and observed again.…
Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons, such as knowing the causative agent of disease, knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study. This study allows the student to exercise their ability to use and understand sterile techniques, transmission, and prevention of microorganisms.…
4. The goal of the direct fluorescent antibody test is to test for the presence of…