Bead Mill Yeast
9.1 Bead Mill Homogenizers
Bead mills is based on the fast stirring of a thickened suspension of microorganisms in the presence of beads. The disruption happens by the crushing action of the glass beads, as the bead hit with the cells. It is considered the best way for disruption for spores, yeast and fungi and works well with tough-to disrupt cells such as cyanobacteria, mycobacteria, spores and microalgae (Hopkins 1991).
The size of the beads is vital. Optimal size for bacteria and spores is 0.1, while yeast, mycelia, microalgae, and unicellular animal cells, such as leucocytes or trypsin zed tissue culture cells is 0.5 mm and 1.0 or 2.5 mm for tissues. Speed of disruption is increased about 50% by using zirconia silica or zirconia rather
Bead mills is based on the fast stirring of a thickened suspension of microorganisms in the presence of beads. The disruption happens by the crushing action of the glass beads, as the bead hit with the cells. It is considered the best way for disruption for spores, yeast and fungi and works well with tough-to disrupt cells such as cyanobacteria, mycobacteria, spores and microalgae (Hopkins 1991).
The size of the beads is vital. Optimal size for bacteria and spores is 0.1, while yeast, mycelia, microalgae, and unicellular animal cells, such as leucocytes or trypsin zed tissue culture cells is 0.5 mm and 1.0 or 2.5 mm for tissues. Speed of disruption is increased about 50% by using zirconia silica or zirconia rather