Abstract:
The Beer’s law lab was conducted to determine the optimal wavelength of Co(NO3)2·6H2O with the use of spectrometry. The results determined that the optimal wavelength to study the absorbance of this salt was 500nm. It also demonstrated how transmittance of light and absorbance of light are inversely proportional because absorbance is calculated by multiplying transmittance by a negative log.
Introduction:
When one is studying chemicals, there are many important factors of significance. The color of a chemical is a useful tool in its study. The light one sees produced by a chemical is the result of both reflection and absorbance of wavelengths. The wavelengths that are absorbed by a chemical are not visualized. The wavelengths that are reflected back are the colors that one sees. When chemicals are diluted in water, their colors also become diluted. As the chemical is diluted, the molecules spread apart. The more dilute the solution, the further apart the molecules. As the molecules spread, the color that is reflected becomes less intense because some of the wavelengths are able to pass through the solution without encountering any of the solute. The more wavelengths that are able to pass through a solution without encountering any of the solute, the greater the transmittance. The transmittance can be mathematically calculated by dividing the amount of light that exited the solution (IT) by the amount of original intensity (IO). That value is then multiplied by 100 to give the percent transmittance (%T) Beer’s Law is used to relate and compares the amount of light that has passed through something to the substances it has passed through. The Law is represented by A=abc. “A” is the absorbance of a solution. The “a” represents the absorption constant of the solution being tested. The “b” represents the thickness of the solution in centimeters, and “c” represents the solution’s molarity or concentration. The