Bio practical

Title : Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions
Objective : To investigate the action of saliva and 3M hydrochloric acid in two carbohydrate solutions
Results :
Table 1:

Observations
Conclusions

Solution A
Benedict’s test : Initial blue solution changed to brick-red precipitate. Little amount of brick-red precipitate suspended in solution. The solution was translucent.

Iodine test : Yellowish-brown remained the same. Abundant amount of yellowish-brown colour remained on the white tile. It was opaque.

Presence of reducing sugar , absence of starch

Solution B
Benedicts test: Blue colour remained the same. Abundant amount of blue solutions remained the same and it was opaque.

Iodine test: Yellowish-brown colour turned to blue-black. Moderate amount of blue-black precipitate was left on the white tile. It was opaque.

Absence of reducing sugar , presence of starch

Table 2 :

Tube

Contents

Temp
(℃)
Benedict’s Test – Colour Observation

After 5th min
(from test tube 1-4 into 1’-4’)
After 35thmin
(from test tube 1-4 into 1’-4’)
1
10ml solution B 1ml saliva
37
Initial blue solution turned to orange. It was opaque.
Initial blue solution turned to orange. It was opaque.
2
10ml solution B 1ml 3M HCl
37
Colour of solution remained blue. It was transparent.
Colour of solution remained unchanged. It was transparent.
3
10ml solution B 1ml 3M HCl
95
Colour of solution remained blue. It was transparent.
Initial blue solution turned to brown. It was translucent.
4
10ml solution B 1ml saliva
95
Initial blue solution changed to green. It was opaque.
Initial blue solution changed to dark green. It was translucent.

Discussion :
1. Name of enzyme involed : The name of enzyme involved in this experiment is salivary amylase, which presents in our saliva.
2. Specific action(s) of enzyme involved : Salivary amylase catalyses the breakdown of complex sugar, such