BIO20002 Prac Report 2 1 
BIO20002 The Microbial World
Practical Report 2
ENVIRONMENTAL PARAMETERS OF GROWTH & BIOSYNTHESIS AND NUTRITION
Due date: 1/5/2015
Name: Hong Thinh Le Student Number: 1717766
Prac class (day and time): Friday / 11:30 am
AIMS
This experiment is aimed to examine the effects of environment such as Oxygen, Temperature, pH and Osmotic Limitations on the growth of a various kind of bacteria.
RESULTS
1. Oxygen Requirements
Depict the pattern of growth of the organisms in the thioglycollate broth and state the type of growth (aerobic, anaerobic or facultatively anaerobic).
E. coli P. fluorescens L. bulgaricus C. butyricum
2. Temperature Range of Growth Score growth using - for no growth and +1 to +4 for growth.
Temperature
Organism
4°C
25°C
37°C
45°C
E. coli
-
+4
+4
+1
P. fluorescens
+3
+4
-
-
B. subtilis
-
+2
+4
+4
3. pH Range of Growth
Score growth using - for no growth and +1 to +4 for growth.
pH
Organism
4
5
6
7
9
E. coli
-
+1
+2
+2
+3
P. fluorescens
-
-
+1
+2
+3
L. bulgaricus
-
-
+1
+1
+2
S. griseus
-
-
+3
+4
+4
S. cerevisiae
+4
+4
+4
+4
+4
4. Osmotic Limitations
Sucrose concentration
Organism
0%
15%
30%
P. fluorescens
+4
+4
+2
S. salivarius
+2
+3
-
S. cerevisiae
+2
+4
+3
5. Fill in the spaces in the following chart with a + or -. For the mineral salts results, score growth using - for no growth and +1 to +4 for growth.
Test
E.coli
K.pneumoniae
S.epidermidis
M.luteus
Glucose
Fermentation:
acid gas
+
+
+
-
Mineral salts plus glucose
+2
+3
+3
+2
Lactose
Fermentation: Acid gas
+
+
+
-
Mineral salts plus lactose
+2
+4
+3
+2
Sucrose
Fermentation: acid gas
-
+
+
-
Mineral salts plus sucrose
+1
+4
+2
+2
Tryptophanase
(Indole Test)
+
-
-
-
Methyl Red
Test
+
-
+
-
Voges-Proskauer
Test
-
+
+
-
DISCUSSION
1. Oxygen Requirement
E.coli was found facultative anaerobe because the growth was seen throughout the middle but was not near the
Practical Report 2
ENVIRONMENTAL PARAMETERS OF GROWTH & BIOSYNTHESIS AND NUTRITION
Due date: 1/5/2015
Name: Hong Thinh Le Student Number: 1717766
Prac class (day and time): Friday / 11:30 am
AIMS
This experiment is aimed to examine the effects of environment such as Oxygen, Temperature, pH and Osmotic Limitations on the growth of a various kind of bacteria.
RESULTS
1. Oxygen Requirements
Depict the pattern of growth of the organisms in the thioglycollate broth and state the type of growth (aerobic, anaerobic or facultatively anaerobic).
E. coli P. fluorescens L. bulgaricus C. butyricum
2. Temperature Range of Growth Score growth using - for no growth and +1 to +4 for growth.
Temperature
Organism
4°C
25°C
37°C
45°C
E. coli
-
+4
+4
+1
P. fluorescens
+3
+4
-
-
B. subtilis
-
+2
+4
+4
3. pH Range of Growth
Score growth using - for no growth and +1 to +4 for growth.
pH
Organism
4
5
6
7
9
E. coli
-
+1
+2
+2
+3
P. fluorescens
-
-
+1
+2
+3
L. bulgaricus
-
-
+1
+1
+2
S. griseus
-
-
+3
+4
+4
S. cerevisiae
+4
+4
+4
+4
+4
4. Osmotic Limitations
Sucrose concentration
Organism
0%
15%
30%
P. fluorescens
+4
+4
+2
S. salivarius
+2
+3
-
S. cerevisiae
+2
+4
+3
5. Fill in the spaces in the following chart with a + or -. For the mineral salts results, score growth using - for no growth and +1 to +4 for growth.
Test
E.coli
K.pneumoniae
S.epidermidis
M.luteus
Glucose
Fermentation:
acid gas
+
+
+
-
Mineral salts plus glucose
+2
+3
+3
+2
Lactose
Fermentation: Acid gas
+
+
+
-
Mineral salts plus lactose
+2
+4
+3
+2
Sucrose
Fermentation: acid gas
-
+
+
-
Mineral salts plus sucrose
+1
+4
+2
+2
Tryptophanase
(Indole Test)
+
-
-
-
Methyl Red
Test
+
-
+
-
Voges-Proskauer
Test
-
+
+
-
DISCUSSION
1. Oxygen Requirement
E.coli was found facultative anaerobe because the growth was seen throughout the middle but was not near the
References: “Gas gangrene”, MedlinPlus Trusted Helth Information for you, < http://www.nlm.nih.gov/medlineplus/ency/article/000620.htm> “Oxidative stress response in Clostridium perfringens”, microbiology < http://mic.sgmjournals.org/content/150/6/1649.full>