Biocaltcau Reaction Lab Report

The total protein content was estimated as proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2, 0.4, 0.6, 0.8, 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes, 0.1 and 0.2 ml of the extract were taken. In each test tube, the volume was made up to 1 ml, followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left undisturbed for 10 minutes. Then, 0.5 ml of Folin-Ciocaltcau reagent was added rapidly. They were incubated at room temperature for 30 minutes until a blue colour appeared. Before taking the readings of the standard and the samples, the spectronic