Biofilm-Mediated Enhanced Crude Oil Degradation by
International Research Journal of Environment Sciences________________________________ ISSN 2319–1414 Vol. 2(2), 48-52, February (2013) Int. Res. J. Environment Sci.
Isolation, Characterization and Identification of Diesel Engine Oil Degrading Bacteria from Garage Soil and Comparison of their Bioremediation Potential
Teli Nikhil1, Verma Deepa2, Gavankar Rohan1 and Bhalerao Satish3
1
Department of Biotechnology, Viva College, Virar (W), Maharashtra, INDIA 2 Department of Botany, Viva College, Virar (w), Maharashtra, INDIA 3 Department of Botany, Wilson College, Mumbai, Maharashtra, INDIA
Available online at: www.isca.in
Received 30th November 2012, revised 12th January 2013, accepted 25th January 2013
Abstract
The rate of biodegradation of diesel engine oil by microorganisms isolated from garage soil (petroleum contaminated soil) was studied. Modified diesel engine oil medium was used and two most abundant microorganisms were isolated from garage soil – Micrococcus sp. and Pseudomonas sp. were found to be hydrocarbon degraders and these two bacteria’s were selected for the degradation test. The degradation of diesel engine oil was monitored at a five day interval up to twenty five day period, using gravimetric method. After 25 days of incubation period, Pseudomonas sp. degraded 67.57 % of the oil and Micrococcus sp. with 52.95 %. But the mixture of Micrococcus sp. and Pseudomonas sp. were found to have great potential to degrade diesel engine oil i.e. 89.98 % after 25 days. The rate of degradation of diesel engine oil by Micrococcus sp. was found to be 7.48 x 10-4gm/hr and that of Pseudomonas sp. was 9.55 x 10-4gm/hr while the mixture of both bacterial isolates showed highest rate of degradation of diesel engine oil i.e. 1.27 x 10-3gm/hr. Keywords: Bioremediation, Diesel engine oil, oil spills, hydrocarbon degraders, Micrococcus sp., Pseudomonas sp.
Introduction
As we dig deeper into the modern industrial age of technologies, several aspects of
Isolation, Characterization and Identification of Diesel Engine Oil Degrading Bacteria from Garage Soil and Comparison of their Bioremediation Potential
Teli Nikhil1, Verma Deepa2, Gavankar Rohan1 and Bhalerao Satish3
1
Department of Biotechnology, Viva College, Virar (W), Maharashtra, INDIA 2 Department of Botany, Viva College, Virar (w), Maharashtra, INDIA 3 Department of Botany, Wilson College, Mumbai, Maharashtra, INDIA
Available online at: www.isca.in
Received 30th November 2012, revised 12th January 2013, accepted 25th January 2013
Abstract
The rate of biodegradation of diesel engine oil by microorganisms isolated from garage soil (petroleum contaminated soil) was studied. Modified diesel engine oil medium was used and two most abundant microorganisms were isolated from garage soil – Micrococcus sp. and Pseudomonas sp. were found to be hydrocarbon degraders and these two bacteria’s were selected for the degradation test. The degradation of diesel engine oil was monitored at a five day interval up to twenty five day period, using gravimetric method. After 25 days of incubation period, Pseudomonas sp. degraded 67.57 % of the oil and Micrococcus sp. with 52.95 %. But the mixture of Micrococcus sp. and Pseudomonas sp. were found to have great potential to degrade diesel engine oil i.e. 89.98 % after 25 days. The rate of degradation of diesel engine oil by Micrococcus sp. was found to be 7.48 x 10-4gm/hr and that of Pseudomonas sp. was 9.55 x 10-4gm/hr while the mixture of both bacterial isolates showed highest rate of degradation of diesel engine oil i.e. 1.27 x 10-3gm/hr. Keywords: Bioremediation, Diesel engine oil, oil spills, hydrocarbon degraders, Micrococcus sp., Pseudomonas sp.
Introduction
As we dig deeper into the modern industrial age of technologies, several aspects of
References: 1. Hill G.B., Moxey J.G., Gasoline and Diesel oil In: Gathee VB (ed) Petroleum Product Handbook Mc-Grew Hill, 4, 1-4 NY (1980) 11. Ijah U.J.J., Okang C.N., Petroleum Degrading capabilities of bacteria isolated from soil, W.A.J. Biol. Appl. Chem., 38(1-4), 915 (1993) International Science Congress Association 52