Carbohydrates, Proteins Lipids
And Nucleic Acids
Professor Anthony Depass
Biology 1
9/25/14
Introduction:
By completing lab exercise 6 : Biologically Important Molecules, we are able to perform tests such as the Benedicts Test for Reducing Sugars, Iodine Test, Biuret Test, Sudan IV Test, and lastly the Grease-Spot Test that detect the presence of biologically important carbohydrates, protein lipids and nucleic acids. Most known compounds in living organism are if fact carbohydrates, lipids, proteins, and nucleic acids. Each of these macromolecules are tested differently. The Benedicts test identifies reducing sugars based on their ability to reduce cupric ions to cuprous oxide at basic pH resulting in a green to reddish orange color. The basis for the iodine test for starch is since starch is a coiled polymer of glucose, the iodine interacts with …show more content…
While one solutions changed color to green or a reddish brown color, others did not. Since the Onion juice, glucose solution and the reducing sugar solution changed color to either green or dark red color. this is proof that the solutions where able to change cupric ions into cuprous oxide. The other solutions such as the water potato juice and so on did not react to the Benedict’s reagent meaning they did not have free aldehydes or ketones with their solutions. In the Iodine test for starch, lonely two of the substance reacted with the iodine. The potato juice and the starch solution turn to a dark blackish color when the iodine was added shows that there are coiled carbohydrates that are with the juice and starch solution. As for the solutions and substance that did not react with the iodine that does not meant that there are not carbohydrates with in the solutions only that the carbohydrates are not in a coiled structure, since iodine will not react with carbohydrates unless they are coiled