biology amylase
Biology- Change in pH
Aim: To investigate the affect that change in pH levels has on a particular enzyme, in this case amylase.
Hypothesis: In this investigation I expect as the pH reaches the optimum level, the rate of reaction will be fastest, compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known that the pH of an enzymes environment will affect its activity rate.
Discussion:
In order to identify the dependent and independent variable and describe the difference between them, I will first define what an independent variable is. An independent variable is the variable you have control over and is what you can choose and manipulate. It is usually what you think will affect the dependent variable. In some cases, you may not be able to manipulate the independent variable. It may be something that is already there and is fixed, something you would like to evaluate with respect to how it affects something else, the dependent variable for example colour, kind, time. For this investigation the independent variable would be the pH level. As I have control over it which subsequently has an affect on the dependent variable.
A dependent variable is what you measure in the experiment and what is affected during the experiment. It is called dependent because it ‘depends’ on the independent variable. In an experiment, you cannot have a dependent variable without an independent one. In my investigation the dependent variable would be the time for disappearance of colour as the varying pH levels affect it. Therefore it ‘depends’ on an independent variable for the experiment to work.
The difference between the independent (pH) and dependent (time for disappearance of colour) variables is that the time for disappearance of colour relies on the pH to actually
Aim: To investigate the affect that change in pH levels has on a particular enzyme, in this case amylase.
Hypothesis: In this investigation I expect as the pH reaches the optimum level, the rate of reaction will be fastest, compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known that the pH of an enzymes environment will affect its activity rate.
Discussion:
In order to identify the dependent and independent variable and describe the difference between them, I will first define what an independent variable is. An independent variable is the variable you have control over and is what you can choose and manipulate. It is usually what you think will affect the dependent variable. In some cases, you may not be able to manipulate the independent variable. It may be something that is already there and is fixed, something you would like to evaluate with respect to how it affects something else, the dependent variable for example colour, kind, time. For this investigation the independent variable would be the pH level. As I have control over it which subsequently has an affect on the dependent variable.
A dependent variable is what you measure in the experiment and what is affected during the experiment. It is called dependent because it ‘depends’ on the independent variable. In an experiment, you cannot have a dependent variable without an independent one. In my investigation the dependent variable would be the time for disappearance of colour as the varying pH levels affect it. Therefore it ‘depends’ on an independent variable for the experiment to work.
The difference between the independent (pH) and dependent (time for disappearance of colour) variables is that the time for disappearance of colour relies on the pH to actually