Biology Digestive Enzyme Experiment
Biology Digestive Enzyme Experiment
“Design an experiment in which you will investigate a digestive enzyme’s effect on digestion”
Research Question:
To determine the effect of enzyme concentration on the rate of digestion. This will be done by increasing the concentration of the enzyme diastase and investigate its effect on the rate of starch digestion. The rate will be determined by the amount of time it takes to completely digest the powdered starch, the complete digestion will be indicated by the color change of the solution by iodine.
Hypothesis:
My hypothesis is that an increase in enzyme concentration will cause the faster digestion of starch. This means that it will increase the rate of starch digestion.
Background information explains that substrates bind to the active site of an enzyme by random movement of the molecules in liquids. An increase in enzyme concentration will increase the frequency of collisions that takes place between the substrates and enzymes, forming more substrate-enzyme complexes in a shorter time, meaning breaking down substrates in a shorter amount of time. So we see here how an increase in the concentration of the enzyme diastase, will increase the rate of starch digestion. Though the amount of substrates can be a limiting factor, in that an increase in enzyme concentration will not cause any further increase in the rate of digestion, as there is no more substrate to be digested.
Predictive Graph Sketch:
This is the predictive graph sketch of the effect of concentration of the enzyme diastase on the rate of starch digestion.
Variables:
Independent Variable:
- Diastase enzyme concentration
The precision in the increasing of concentration of the diastase enzyme will be kept by ensuring the appropriate amount of powdered diastase dissolved in 100 ml of distilled water at controlled increments (1 gram increments from 1-5 grams).
Dependent Variable:
- Time it takes for starch to be completely digested
“Design an experiment in which you will investigate a digestive enzyme’s effect on digestion”
Research Question:
To determine the effect of enzyme concentration on the rate of digestion. This will be done by increasing the concentration of the enzyme diastase and investigate its effect on the rate of starch digestion. The rate will be determined by the amount of time it takes to completely digest the powdered starch, the complete digestion will be indicated by the color change of the solution by iodine.
Hypothesis:
My hypothesis is that an increase in enzyme concentration will cause the faster digestion of starch. This means that it will increase the rate of starch digestion.
Background information explains that substrates bind to the active site of an enzyme by random movement of the molecules in liquids. An increase in enzyme concentration will increase the frequency of collisions that takes place between the substrates and enzymes, forming more substrate-enzyme complexes in a shorter time, meaning breaking down substrates in a shorter amount of time. So we see here how an increase in the concentration of the enzyme diastase, will increase the rate of starch digestion. Though the amount of substrates can be a limiting factor, in that an increase in enzyme concentration will not cause any further increase in the rate of digestion, as there is no more substrate to be digested.
Predictive Graph Sketch:
This is the predictive graph sketch of the effect of concentration of the enzyme diastase on the rate of starch digestion.
Variables:
Independent Variable:
- Diastase enzyme concentration
The precision in the increasing of concentration of the diastase enzyme will be kept by ensuring the appropriate amount of powdered diastase dissolved in 100 ml of distilled water at controlled increments (1 gram increments from 1-5 grams).
Dependent Variable:
- Time it takes for starch to be completely digested