Kenishia Pascal 10x3.
Investigating How Different Concentration Effects The Rate Of Reaction.
Strategy A
Possible Factors
* Source of catalase * Concentration * Surface Area of enzyme * Concentration of enzyme * pH * Temperature
Chosen Factor
We chose to investigate the concentration of enzyme as we had previously investigated the optimum temperature for catalase in the preliminary investigation. Concentration of enzyme is also fairly easy to investigate, as you need to only increase the amount of potato that you want to investigate at a given time.
Hypothesis
As the concentration of enzyme increases it will have an effect on the rate of reaction. The suggested optimum will be 10g as the higher the mass of catalase the more enzymes, meaning more space for reactions to occur. In the space given there will be more enzymes reacting with the substrate, which in this experiment is hydrogen peroxide (H2O2), until it has reached its optimum, where the volume of oxygen produced will not increase due to the fact that there will not be enough H2O2 left for the enzymes
Quantitative Hypothesis
By doubling the mass of potato, the rate of reaction will double also.
2n=2r
Scientific Explanation
Enzymes are made from amino acids, and which are proteins. When an enzyme is formed; it is made by stringing together between 100 and 1,000 amino acids in a very unique order, the chain of amino acids then folds into a unique shape. That shape allows the enzyme to carry out specific chemical reaction
“Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being used up”. The place where these substrate molecules fit is called the active site.
Enzymes are proteins produced by micro-organisms, in turn speeding up the chemical reactions. These can be found in the cell’s gene. The lock and key model is a key way to express the way in