blood lab
Nick Work
October 20, 3013
CSI: Intro to Biology
Blood Sampling
Objective
Our Objective is to identify which blood type each sample is. We test Anti-A and Anti – B, and also test to determine if it is Rh positive or negative.
Hypothesis
We are testing unknown blood sample testing for what type of blood. When A is shown in the blood sample agglutination will accrue causing it to look grainy.
Materials
At least 4 dropper vial
3 blood samples
Synthetic Anti – A
Synthetic Anti –B
Synthetic Anti – Rh serum
3 tooth picks (to stir)
Blood typing side
Methods
Using the dropper vial, place a drop of the first synthetic blood sample in each well of the blood typing slide. Then add a drop of Anti-A to one well labeled A. Next add a drop of Anti-B to another well labeled B. Next we added a drop of Anti-Rh serum to the last well labeled, RH. Using different color mixing sticks for each well. If a film remains uniform in appearance, here is no agglutination. If the sample appears granular, agglutination has occurred. A positive agglutination reaction indicates blood type.
Results
In our results we found that our blood sample tested positive for Anti-A and also for Anti-B. Our Rh tested negative. Therefore we are left with enough evidence to conclude our blood type. Our sample was AB-.
Conclusion
In conclusion by figuring out what our blood type was, we were able to learn how to test for all blood types. We learned that blood type B+ that shows agglutination for Anti-B serum and also for Rh. And we also learned about blood type O+ that only showed agglutination from Rh serum.
October 20, 3013
CSI: Intro to Biology
Blood Sampling
Objective
Our Objective is to identify which blood type each sample is. We test Anti-A and Anti – B, and also test to determine if it is Rh positive or negative.
Hypothesis
We are testing unknown blood sample testing for what type of blood. When A is shown in the blood sample agglutination will accrue causing it to look grainy.
Materials
At least 4 dropper vial
3 blood samples
Synthetic Anti – A
Synthetic Anti –B
Synthetic Anti – Rh serum
3 tooth picks (to stir)
Blood typing side
Methods
Using the dropper vial, place a drop of the first synthetic blood sample in each well of the blood typing slide. Then add a drop of Anti-A to one well labeled A. Next add a drop of Anti-B to another well labeled B. Next we added a drop of Anti-Rh serum to the last well labeled, RH. Using different color mixing sticks for each well. If a film remains uniform in appearance, here is no agglutination. If the sample appears granular, agglutination has occurred. A positive agglutination reaction indicates blood type.
Results
In our results we found that our blood sample tested positive for Anti-A and also for Anti-B. Our Rh tested negative. Therefore we are left with enough evidence to conclude our blood type. Our sample was AB-.
Conclusion
In conclusion by figuring out what our blood type was, we were able to learn how to test for all blood types. We learned that blood type B+ that shows agglutination for Anti-B serum and also for Rh. And we also learned about blood type O+ that only showed agglutination from Rh serum.