Bottle A Case Study

The bacteria were re-suspended by transferring 10-12 mL of bacterial growth media (Bottle A) to the dried bacteria (Bottle B). The Bottle B was covered with Aluminum foil and roughly mixed for 30 seconds. 100 μL from bacterial suspension transferred to a new bacterial growth medium (Bottle A). The suspension was mixed by inverted and incubated at 37◦ C overnight for 8-12 hours. The density of bacterial cells was measured by spectrophotometer at OD600, which was around