C Testosteroni Case Study

The strain of C. testosteroni bdq06 (NCBI No. KM277791) was isolated from active sludge in the
86 wastewater treatment plant of Jilin Petrochemical Company (China) and cultured in a mineral medium as
87 described previously with quinoline as the sole carbon, nitrogen and energy source(Geng, et al., 2015). It
88 has the ability to completely degrade quinoline into carbon dioxide and water, and convert nitrogen in
89 pyridine ring into ammonia, nor into nitrite(Geng, et al., 2015).
90
91 2.2. Reactor operation
92 The accidental wastewater, stored in an emergency overflow pond of the plant, was taken as raw
93 wastewater for treatment. The property of water was shown in Table 1. It was characterized as reddish
94 brown color with low pH, containing
The strain of C. testosteroni bdq06 (5 g, wet weight) was inoculated to Reactor A at the Stage
110 2 to compare the bioaugmentation effects with Reactor B with inoculating the same weight of seed sludge.
111
112 At the beginning of the operation, the HRT and sludge retention time (SRT) were kept for 6 hours and 15 days respectively. The mixed liquor suspended solids (MLSS) was controlled at 3500±300mg l
-1
113 by
114 adding seed sludge which collected from the same Municipal Plant, and then the reactors were aerated at the rate of 0.5 l min-1
115 controlled by a spinner flowmeter (LZT-6M, JuEnviron Company) followed by
116 pumping the mixed wastewater into the two reactors by a two-channel peristaltic pump (HL-2B, Shanghai
Huxi Company) at the rate of 2.56 ml min-1
. The temperature was maintained at 15±1 o 117 C in
118 correspondence with the domestic wastewater treatment plant operating conditions. The samples from the
119 influent, effluent and reactors were taken for analysis.
120
121 2.3. Analysis methods
122 A high performance liquid chromatography (LC-20A, Shimazu) coupled with a C18 analytical column (4.6
123 mm × 150mm, 5 μm particle size) was used to determine the concentration of
The concentration of NH4
+
128 was determined using a cation column (C4-100,
Metrohm AG) while NO3
-
, SO4
2-
and NO2
-
129 were analyzed with an anion column (Supp4-250, Metrohm AG).
130 Total organic carbon (TOC) was analyzed by using a TOC analyzer (TOC 5000, Shimdzu). The samples
131 were filtrated by using a filter membrane (0.45μm, EMD Millipore) before analysis and all samples were
132 analyzed twice.
133
134 At the end of Stage 4, 1 liter of the effluent was collected and extracted by methylene dichloride into acid,
135 base and neutral fractions, and was compared with those extracted from the same volume of raw
6
136 wastewater. The extract was enriched to 1 ml with a termovap sample concentrator (SC-2800D, ChromTech)
137 and 2 μl of the concentrated extract was qualitatively analyzed using GC/MS (QP2010 Ultra, Shimazu) to
138 determine the organic constituents in the samples according to Li’s method(Li, et al., 2003). The sample
139 was analyzed at splitless mode using MS ion source. The carrier gas was ultrapure helium with a constant-flow rate of 1.3 ml