As we can see the particles size was varied by varying the polymer ratio, and the hydrophobic moiety immobilized on the COS chain. For the COS, and its oleoyl-CGKRK derivatives, the complexes showed increase in the size by increasing the polymer ratio from 1 to 5 folds. Increasing the polymer ratio to 10 folds resulted in a decrease in the size. Meanwhile, in the case of saturated fatty acids including the palmitoyl-CGKRK, and stearoyl-CGKRK derivatives of chitosan, they exhibited different pattern as the polymer: siRNA weight ratio increases from 1 to 5 the particle size decrease, then it increased when the weight ratio of polymer: siRNA reached 10. Additionaly, we noticed that among the chitosan derivatives oleiyl derivative had the smallest particle size at all polymer ratios. It is clear that, the fatty acid chain and its degree of hydrophilicity had a strong impact on the polymer/siRNA complexes size, figure
As we can see the particles size was varied by varying the polymer ratio, and the hydrophobic moiety immobilized on the COS chain. For the COS, and its oleoyl-CGKRK derivatives, the complexes showed increase in the size by increasing the polymer ratio from 1 to 5 folds. Increasing the polymer ratio to 10 folds resulted in a decrease in the size. Meanwhile, in the case of saturated fatty acids including the palmitoyl-CGKRK, and stearoyl-CGKRK derivatives of chitosan, they exhibited different pattern as the polymer: siRNA weight ratio increases from 1 to 5 the particle size decrease, then it increased when the weight ratio of polymer: siRNA reached 10. Additionaly, we noticed that among the chitosan derivatives oleiyl derivative had the smallest particle size at all polymer ratios. It is clear that, the fatty acid chain and its degree of hydrophilicity had a strong impact on the polymer/siRNA complexes size, figure