Ca-2pb2 Synthesis Lab Report

In HEPES buffer at pH 7.0, the CA-2Pb2+ formation resulted in a distinct color change from red to blue, accompanied by a remarkable bathochromic shift from 527 nm with a shoulder at 558 nm to 605 nm in the absorption spectrum [28]. Since there have been some reports on the complex formation between Cys and Pb2+ in an aqueous solution [30, 31], the sensing selectivity of CA-2Pb2+ toward Cys was investigated by absorption spectroscopy. The color changes and absorption spectral study of CA-2Pb2+ (50 μM) were performed upon addition of 21various amino acids and peptide (100 μM) such as Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Hcy, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val, and GSH in 10 mM HEPES buffer solution at pH 7.0 (Fig. 1).