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Cervical Cancer

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Cervical Cancer
Research II
Summarized Project Proposal

Title: The Efficacy of Lubi-lubi (Atropa belladonna) Fruit Extract as Anti-neoplastic Agent against Human Papilloma Virus Related Cervical Neoplasms
Problem: Is the Lubi-lubi extract a potent anti-neoplastic agent against HPV related cervical neoplasms?

Specific Problems: 1. What stage in the maturity of the deadly nightshade fruit is most efficient as an anti-neoplastic agent? 2. What concentration of the extract is most efficient against HPV related cervical neoplasms? 3. What are the implications by the study should the crude polysaccharides derived from the product be proven a potent anti-neoplastic agent?

HYPOTHESES:
Is the Lubi-lubi extract a potent anti-neoplastic agent against HPV related cervical neoplasms?

• Null hypothesis

The lubi-lubi (Atropa belladonna) extract is not a potent anti-neoplastic agent against HPV related cervical neoplasms.

• Operational hypothesis The lubi-lubi (Atropa belladonna) extract is a potent anti-neoplastic agent against HPV related cervical neoplasms.

METHODOLOGY
Materials:

1) For extracting the polysaccharide a) Lubi-lubi (Atropa belladonna) fruit in different levels of maturity b) distillation apparatus c) beakers d) petri dish e) refrigerator (for preservation of samples) f) mortar and pestle g) thermometer 2) for preperation and treatment of cancer cells a) sample of blood with HPV related neoplasms, 5ml per sample b) sample of normal blood, 5ml per sample c) centrifuge d) test tubes e) petri dish f) dropper g) refrigerator (for preservation of samples) h) compound microscope

PROCEDURE

1. Collection of Lubi-lubi fruit - The plant to be used naturally grows in the province of Albay so collecting samples would not be hard.
- To be collected are fruits in different levels of maturity: young, half ripe and
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