ChemiCal Basis of
Chemical Basis of
Life – Part I Carbohydrates
H a n d s - o n l a b s , i n c . Ve r s i o n
42-0144-00-01
LAB REPORT
PHOTOS – Include two digital photos with your lab report, either as separate attachments to an e-mail or paste into your document.
1. Photo #1 – Take a photo of your finished experiment in exercise 2, testing for sugars.
(The 3 test tubes after heating, but while still in the pan.)
Be sure to take a view that shows the colors in all the tubes
^Picture is A, B, X in that order^
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2. Photo #2 – Take a photo of the tubes after you have added the iodine solution.
(Again, be sure that you are close enough to see the results clearly.) …show more content…
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3. Photo #3 – Take a photo of the tubes after testing your cereal – Benedict’s and IKI tests.
^^ The left picture is A-C and right picture is D-F backwards^^
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Exercise 2: Simple Sugars
Observations:
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Data Table 2: Benedict’s reagent test for sugar
Test Tubes
A
B
Initial clear Color
Color After orange
5
X
Was clear
faint yellow
light blue
violet
Questions:
A. What was the initial color of the Benedict’s reagent? How did this compare to the color after heating? The initial color of the benedict reagent in most of the solutions were relatively clear, and bendict reagent itself was blue. After heating there were a range of colors depending on the solution, but the colors were drastically different; www.LabPaq.co !3
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very vivid.
B. Is glucose present in the unknown solution? Explain your answer.
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No, because after heating the unknown solution developed a purple color which is not one of the indicators. C. What are some medical conditions in which it is necessary to monitor glucose levels?
Some medical conditions which involves glucose monitoring is: Diabetes and Hypoglycemia.
D. Could the procedure you conducted in this exercise be classified as a quantitative test?
Yes it can, because we were seeing if the different substances showed any amounts of monosaccharides or disaccharides.
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Exercise 3: Complex Carbohydrates
Observations:
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Data Table 3: Color change in test tubes with IKI solution iKi test for starch Initial Color
Final Color
A
B
X
cloudy clear
clear
clear
very dark blue
orangy-yellow reddish orange/yellow
Questions:
A. Is there starch in the unknown solution? Explain your answer.
There is no starch in the unknown fluid because the colors that came up were not blue which is the indicator when using iodine.
B. Why may it be important to test for starch?
Its important to test for starch because thats a very important energy source.
C. What types of food may be composed of starch?
Its a carbohydrate so its most in foods like: cereal, bread, oats, and other staple foods.
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Exercise 5: Testing Breakfast Cereal
Observations:
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Material tested
Data Table 5: Cereal testing results biuret reagent
Benedict’s reagent
iKi
Cereal
OMIT
yellowish
turned dark blue
dH2o
OMIT
lighter blue
became orange
Questions:
Name of cereal tested: cheerios
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A. Which macromolecules were present in the cereal?
The substances present in the cereal were starch and glucose.
B. Compare your findings to the nutritional information found on the product label. Do the results agree with the labeling? If not, what could be a source of error? Yes it does, because they did list that it has corn starch, and the cereal definitely has sugar.
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