Chemistry Investigatory Project
CHEM. RES. CHINESE UNIVERSITIES 2012, 28(3), 415—418
Determination of Nicotine in Tobacco by Capillary Electrophoresis with Electrochemical Detection
SUN Jin-ying1, XU Xiao-yu1,2, YU Huan1 and YOU Tian-yan1*
1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China; 2. Ministry of Public Security of Jilin Province, Changchun 130051, P. R. China Abstract A sensitive, simple and low-cost method based on capillary electrophoresis(CE) with electrochemical(EC) detection at a carbon fiber microdisk electrode(CFE) was developed for the determination of nicotine. Effects of detection potential, concentration and pH value of the phosphate buffer, and injection time as well as separation voltage were investigated. Under the optimized conditions: a detection potential of 1.20 V, 40 mmol/L phosphate buffer(pH 2.0), a sample injection time of 10 s at 10 kV and a separation voltage of 16 kV, the linear range obtained was from 5.0×10–7 mol/L to 1.0×10–4 mol/L with a correlation coefficient of 0.9989 and the limit of detection(LOD, S/N=3) obtained was 5.0×10–8 mol/L. The method was also used to determine the nicotine in cigarettes. Nicotine amount ranged from 0.211 mg/g to 0.583 mg/g in the pipe tobacco of seven brands of cigarette and the amount in one cigarette varied from 0.136 mg/cigarette to 0.428 mg/cigarette. Keywords Capillary electrophoresis; Electrochemical detection; Nicotine; Tobacco Article ID 1005-9040(2012)-03-415-04
1
Introduction
Nicotine accounts for about 98%(mass fraction) of the total alkaloids and presents in a concentration of 0.5%―8% (mass fraction) in tobacco[1,2]. And nicotine addiction is related with higher risk for many kinds of diseases such as Alzheimer’s, Parkinson’s and even suicide[3,4]. Thus it’s necessary to control nicotine amount in tobacco products. Determination of nicotine is very important in both the tobacco industry and toxicology
Determination of Nicotine in Tobacco by Capillary Electrophoresis with Electrochemical Detection
SUN Jin-ying1, XU Xiao-yu1,2, YU Huan1 and YOU Tian-yan1*
1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China; 2. Ministry of Public Security of Jilin Province, Changchun 130051, P. R. China Abstract A sensitive, simple and low-cost method based on capillary electrophoresis(CE) with electrochemical(EC) detection at a carbon fiber microdisk electrode(CFE) was developed for the determination of nicotine. Effects of detection potential, concentration and pH value of the phosphate buffer, and injection time as well as separation voltage were investigated. Under the optimized conditions: a detection potential of 1.20 V, 40 mmol/L phosphate buffer(pH 2.0), a sample injection time of 10 s at 10 kV and a separation voltage of 16 kV, the linear range obtained was from 5.0×10–7 mol/L to 1.0×10–4 mol/L with a correlation coefficient of 0.9989 and the limit of detection(LOD, S/N=3) obtained was 5.0×10–8 mol/L. The method was also used to determine the nicotine in cigarettes. Nicotine amount ranged from 0.211 mg/g to 0.583 mg/g in the pipe tobacco of seven brands of cigarette and the amount in one cigarette varied from 0.136 mg/cigarette to 0.428 mg/cigarette. Keywords Capillary electrophoresis; Electrochemical detection; Nicotine; Tobacco Article ID 1005-9040(2012)-03-415-04
1
Introduction
Nicotine accounts for about 98%(mass fraction) of the total alkaloids and presents in a concentration of 0.5%―8% (mass fraction) in tobacco[1,2]. And nicotine addiction is related with higher risk for many kinds of diseases such as Alzheimer’s, Parkinson’s and even suicide[3,4]. Thus it’s necessary to control nicotine amount in tobacco products. Determination of nicotine is very important in both the tobacco industry and toxicology
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