Chloramphenicol 101 Lab Report

In this experiment, we observed the effects of four antibiotics against S.aureus and E.coli. The first antibiotic used was Tetracycline. This agent binds to the 30s subunit and blocks the attachment of tRNA. This inhibits the synthesis of proteins. Penicillin is the second antibiotic that inhibits cell wall synthesis (integrity). This is done by the inhibition of trans peptidase which is responsible for catalyzing peptidoglycan. In a gram positive organism this is destructive due to the damaging of the wall and cytoplasm, causing leakage. Chloramphenicol is the third antibiotic, which inhibits protein synthesis in both bacteria and the mitochondria. It does so by blocking the peptidyl transfer (step 50s subunit). With this step inhibited the