Chromatography of Food Dye
MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGY CHEMICAL AND ENVIRONMENTAL TECHNOLOGY DEPARTMENT Lab Report ROOM NO: FE E309
EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes
Submitted by Class Partners Instructor
: Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman
Date lab performed : February 3, 2011 Date of submission : February 10, 2011
FENNELL CAMPUS HAMILTON, ONTARIO
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Purpose:
The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the components of an unknown solution. The practical applications of thin layer chromatography involving food dyes are to find the known food dyes in an unknown solution, which could be useful in safety testing as well as testing for food dyes in terms of allergies.
Procedure:
We began by preparing four small sheets of thin layer chromatography paper, two per person so there would be duplicates of the results. We then pipetted five known dyes and two unknown solutions onto the paper. We then allowed them to develop in the solvent. After the solvent had raised a desirable amount up the chromatography paper we removed it, allowed it to dry and then measured the solvent front, distance to dots and the computed the Rf values.
Data/Observations:
Dyestuff Pink Cochinea Egg Yellow Green Blue Mixture A Mixture B Dyestuff Pink Cochinea Egg Yellow Green Blue Mixture A Table 1 Colour in Solution Colour as a Dried Spot Colour After Development Dark Red Red Dark Red Dark Purple/Red Burgundy Fuchsia Orange-Yellow Orange Orange, Yellow Dark Green Green Blue, Yellow Dark Blue Blue Blue, Pink Dark Red/Orange Yellow Yellow, Pink Dark Red/Brown Brown Red, Blue, Yellow Spots Observed a b c a b c a b a b a b c a b Table 2 Distance to Solvent Front (cm) 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6
2/3
Distance to Spot Center (cm) 0.3 0.9 2.1 0.3 0.9 2.1 0.4 0.9 0.3 0.5 0.4 1 1.3 0.6 1.3
Rf values
EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes
Submitted by Class Partners Instructor
: Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman
Date lab performed : February 3, 2011 Date of submission : February 10, 2011
FENNELL CAMPUS HAMILTON, ONTARIO
1/3
Purpose:
The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the components of an unknown solution. The practical applications of thin layer chromatography involving food dyes are to find the known food dyes in an unknown solution, which could be useful in safety testing as well as testing for food dyes in terms of allergies.
Procedure:
We began by preparing four small sheets of thin layer chromatography paper, two per person so there would be duplicates of the results. We then pipetted five known dyes and two unknown solutions onto the paper. We then allowed them to develop in the solvent. After the solvent had raised a desirable amount up the chromatography paper we removed it, allowed it to dry and then measured the solvent front, distance to dots and the computed the Rf values.
Data/Observations:
Dyestuff Pink Cochinea Egg Yellow Green Blue Mixture A Mixture B Dyestuff Pink Cochinea Egg Yellow Green Blue Mixture A Table 1 Colour in Solution Colour as a Dried Spot Colour After Development Dark Red Red Dark Red Dark Purple/Red Burgundy Fuchsia Orange-Yellow Orange Orange, Yellow Dark Green Green Blue, Yellow Dark Blue Blue Blue, Pink Dark Red/Orange Yellow Yellow, Pink Dark Red/Brown Brown Red, Blue, Yellow Spots Observed a b c a b c a b a b a b c a b Table 2 Distance to Solvent Front (cm) 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6 3.6
2/3
Distance to Spot Center (cm) 0.3 0.9 2.1 0.3 0.9 2.1 0.4 0.9 0.3 0.5 0.4 1 1.3 0.6 1.3
Rf values