Preview

Chromatography Of Unknown Samples And Paprik Analysis Of Acetaminophen

Better Essays
Open Document
Open Document
990 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Chromatography Of Unknown Samples And Paprik Analysis Of Acetaminophen
Thin Layer Chromatography of Unknown Samples and Paprika
By: Uyen Huynh
Date: June 18, 2014
Lab Partners: Philip Murray
Professor and section: M. Pandey, CHM2210L.601

Data:
Analysis of Drug Store Items
Knowns
Rf Values
Aspirin
.91 cm
Acetaminophen
.85 cm
Ascorbic Acid
.86 cm
Unknown
.97 cm
Identity of unknown mixture
Acetaminophen

Analysis of Paprika Pigments
Spot Number
Rf
Color
1
1
Red-orange
2
1
Red-orange

Calculations A. Analysis of Paprika picture sample
B. Flower sample for paper chromatography
Analysis of Drug Store Items
Knowns
Rf Values= a/f
Aspirin
7.0/7.7= .91 cm
Acetaminophen
7.7/7.7= 1 cm
Ascorbic Acid
6.6/7.7= .86 cm
Unknown
7.5/7.7= …show more content…
We found the Rf value by the distance spot travels divided by the distance the solvent travels. From the TLC samples collected, the solvent travels to be 7.7 cm and the distance the spot travels from aspirin, acetaminophen, ascorbic acid, and unknown to be 7.0 cm, 7.7 cm, 7.6 cm, and 7.5 cm respectively. Since the two …show more content…
Our experiment proved our hypothesis to be somewhat right because we did see most of it without the UV light but there were some parts on towards the middle that we could only see with the UV light which means about half of it was colorless and couldn’t be seen under regular lighting. For the paprika experiment since it had color, we could see all of the spots on the plate under regular lighting and we did try putting it under UV light as it was the same spots seen under regular lighting so we concluded that whatever was colorless from the beginning couldn’t be seen in regular lighting and whatever sample had color could be seen under regular
Continue Reading
View Writing Issues

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Lab: Isolation of Sucrose/Aspirin/Unknown
    • 291 Words
    • 2 Pages

    Lab: Isolation of Sucrose/Aspirin/Unknown

    Isolation of Sucrose: 3.01 g Panacetin were weighed in a 125-mL Erlenmeyer flask, and 51mL dichloromethane were added to partially dissolve the Panacetin. The insoluble portion was gravity filtered and air dried to yield 0.45 g of sucrose (15.0 % of original Panacetin).…

    • 291 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Good Essays

    Crude Acetaminophen Synthesis
    • 860 Words
    • 4 Pages

    Crude Acetaminophen Synthesis

    The crude acetaminophen was expected to be dark in color. This was due to the fact that the impurities were formed from oxidation of the starting phenol. The intensity of this was enough to impart color to the crude acetaminophen.…

    • 860 Words
    • 4 Pages
    Good Essays
    Read More
  • Better Essays

    Tlc Chem 121
    • 3016 Words
    • 13 Pages

    Tlc Chem 121

    2. We can see that dye #3 travelled the least distance during the development process. As a result the Rf value for dye #3 is the smallest of the six. What would cause the dye to travel less than the other dyes?…

    • 3016 Words
    • 13 Pages
    Better Essays
    Read More
  • Good Essays

    Isolation of the Active Ingredient in an Analgesic Drug
    • 783 Words
    • 4 Pages

    Isolation of the Active Ingredient in an Analgesic Drug

    Acetaminaphen was crushed then extracted for the active ingredient by mixing it with methanol. Then separated from the binders by centrifugation and a filtration technique using a Pasteur pipet packed with alumina. The remaining solvent was then evaporated to yield the solid analgesic(.2295g, 45.9% yield) which was collected by filtration and tested for the purity of the drug by the melting point determination. The melting point was (135-142 C) compared to the literature melting point values for acetaminophen (169-171 C) the lower melting point show that their were still impurities in the isolated ingredient.…

    • 783 Words
    • 4 Pages
    Good Essays
    Read More
  • Good Essays

    Tlc questions
    • 567 Words
    • 3 Pages

    Tlc questions

    1. 3-decanone will have a higher Rf value than 3-decanol, toluene will have a higher Rf value than benzoic acid, and cyclooctane will have a higher Rf value than cycloctanone. This is because they are not as polar as the compounds with lower Rf values, the compounds that are most polar tend to stay longer with the polar adsorbent, while the least polar compound travels with the eluent that is lower in polarity.…

    • 567 Words
    • 3 Pages
    Good Essays
    Read More
  • Satisfactory Essays

    Lab for Grade 10 Chem Percentage Yield
    • 258 Words
    • 2 Pages

    Lab for Grade 10 Chem Percentage Yield

    By using a spectrophotometer with the unknown solution, a percent transmittance of 56 appeared. The concentration of the unknown solution was then easily plotted on a graph to show the corresponding data.…

    • 258 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Powerful Essays

    Chem Lab. Identyfing organic solid
    • 862 Words
    • 4 Pages

    Chem Lab. Identyfing organic solid

    In this experiment students were taking the slow melting point of their unknown compound. The tabulated list of all melting points of all students in the lab was created what was very helpful in finding a partner with the same organic solid based on the melting point data. Then with all possible matches students performed mixed melting point and the TLC experiment to find if they have the same retention factor (Rf). At the end of the lab students recorded the infrared spectrum (IR) of an organic compound by using FT-IR spectrometer. After the lab period a H NMR spectrum was given by TA.…

    • 862 Words
    • 4 Pages
    Powerful Essays
    Read More
  • Good Essays

    Tlc Lab
    • 515 Words
    • 3 Pages

    Tlc Lab

    It is important to note that acetic acid is the non-polar component of the experiment. On the other hand, the stationary phase corresponded to the Silicon on the TLC plate. From the dipole in Silicon and Oxygen bonds, this is regarded as the polar component of the experiment. Compared to the mobile phase, the stationary phase results in the polar functional groups to be a stronger attractant. As a result, the most polar functional groups will travel up less the TLC plate. Due to the non-polar compounds not sticking to the stationary phase as strongly as the polar compounds do, non-polar compounds should have a larger Rf value than polar compounds. As previously stated, the least to most polar is Ibuprofen, Acetylsalicylic Acid, Acetaminophen, and Caffeine respectively. This shows that ideally, Caffeine has the lowest Rf value while Ibuprofen should have the largest Rf value. In the Observations section, we calculated that Ibuprofen’s Rf value is .81, Acetylsalicylic Acid’s Rf value is .64, Acetaminophen’s Rf value is .46, and Caffeine’s Rf value is .10. This illustrates that the Rf values trend match the the four analgesics’ polarity…

    • 515 Words
    • 3 Pages
    Good Essays
    Read More
  • Satisfactory Essays

    Tlc Analysis of a Drug Component
    • 356 Words
    • 2 Pages

    Tlc Analysis of a Drug Component

    I measured that the solvent traveled 64mm up the TLC plate by using the starting line as a reference, and, by using that information, I was able to calculate the Rf value of each solution by using:…

    • 356 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Good Essays

    Chem Lab Chromatography
    • 558 Words
    • 3 Pages

    Chem Lab Chromatography

    2. In chromatography, components of a mixture distribute themselves between the stationary phase and the mobile phase. Explain how the components can be separated with these two phases.…

    • 558 Words
    • 3 Pages
    Good Essays
    Read More
  • Powerful Essays

    Aspirin Sample
    • 1996 Words
    • 8 Pages

    Aspirin Sample

    This lab has the following two concepts: synthesis of acetylsalicylic acid and analysis of acetylsalicylic acid. Synthesis is a purposeful execution of chemical reactions to obtain a product. This concept is used in the first part of the lab; when we have to produce crystals of aspirin. Analysis is the separation, identification, and quantification of the chemical components of natural and artificial materials. This concept is used throughout the lab when we are analyzing different reactions that are happing during the duration of the lab.…

    • 1996 Words
    • 8 Pages
    Powerful Essays
    Read More
  • Better Essays

    Coordination Complex Lab
    • 716 Words
    • 3 Pages

    Coordination Complex Lab

    Some of the solution was removed and more deionized water (1.50mL) was added to the solution. A cuvette was filled with deionized water and another with the solution. Each cuvette was filled to the same level. The transmittance of the solution was found at 400nm and then consecutively recorded at intervals of 25nm. Absorbance was calculated from percent transmittance and then plotted on a graph as a function of…

    • 716 Words
    • 3 Pages
    Better Essays
    Read More
  • Better Essays

    Bio Spectroscopy Lab
    • 1273 Words
    • 6 Pages

    Bio Spectroscopy Lab

    This investigation used spectroscopy to evaluate light absorption in different solutions. A spectrophotometer was used in the lab to determine these values. A spectrophotometer is an apparatus used to “measure the absorption of radiation in the visible and UV regions of the spectrum and allows precise at a particular wave length” (Jones et al., 2007). The amount of light absorbed by a substance is directly in relation to the concentration of the solute and also the wavelength moving through the solute (Jones et al., 2007). This is commonly referred to as Beer’s Law and can be expressed as A= εl [C]. Beer’s Law equation measures the absorbency of light, making it an effective measure as spectrophotometers give exact values for absorbency (Jones et al., 2007).…

    • 1273 Words
    • 6 Pages
    Better Essays
    Read More
  • Better Essays

    Kool-Aid Chromatography Lab Report
    • 1375 Words
    • 6 Pages

    Kool-Aid Chromatography Lab Report

    When the actual FD&C dyes in the green Kool-Aid were revealed, it was discovered that the initial assumption that Yellow 5 and Blue 1 are in the Kool-Aid was correct. However, solely based upon the Rf values, the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However, the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because one color band was of a blue tint and the other, a yellow tint. Therefore, based on this qualitative data, the dyes in the green Kool-Aid are Blue 1, which has a an Rf of 0.84, and Yellow 6, which has an Rf value of 0.48. This assumption was made because Yellow 6 has the closest yellow Rf value to the yellow band…

    • 1375 Words
    • 6 Pages
    Better Essays
    Read More
  • Satisfactory Essays

    4-Phenylphenol Errors
    • 487 Words
    • 2 Pages

    4-Phenylphenol Errors

    The proper way to calculate Rf values is the distance your compound traveled divided by the distance your solvent traveled. In other words, you need to measure how far away from your baseline each compound is. You can choose which part of the spot you would like to measure to, either bottom, middle, or top, but once you choose you need to keep that consistent throughout all TLC plates you use for the same reaction. Once you’ve determined how far each compound has traveled you need to determine how far the solvent traveled. The best way to do this is to have a solvent front line close to the top of the TLC plate. From the picture of your TLC plate, I see that you had one; you should make sure all your solvent fronts are the same distance away from the baseline even if one TLC plate is shorter or longer than another. This will also make your calculations easier because your “distance solvent traveled” will be the same for all TLC plates. Once all Rf values are calculated you should see an upward trend the closer to the solvent front you get and a downward trend the closer you get to the baseline. Also, as a precaution, you should always circle the spots made on your TLC plate so you can always see how far they traveled and how intense they were. This will help when all your compounds are colorless and UV lights or an Iodine chamber is needed to view the…

    • 487 Words
    • 2 Pages
    Satisfactory Essays
    Read More

Related Topics