Colligative Properties & Osmotic Pressure
Colligative Properties & Osmotic Pressure (Lab – Chapter 13)
Introduction: In this lab, we are going to observe the difference in freezing point between pure water and salt water. We will also observe the permeability of an egg shell membrane and dialysis tubing, acting in place of a human cell, when placed into a hypertonic or hypotonic solution. We will observe the changes in size, shape, and characteristic.
Materials and Methods: First we begin this experiment by making an ice bath in the 100mL beaker. I filled a test tube with room temperature distilled water and recorded the temperature to be consistent at 23˚C. I put the room temperature test tube in the ice bath and recorded the change in temperature every thirty seconds. After roughly 510 seconds the temperature was consistent at 0˚C. Next I repeated the same experiment, except this time the test tube contained salt water. The temperature in the test tube dropped more quickly and consistently stayed -2˚C after just 330 seconds. I recorded my temperature readings in the table below. Next, I began part two of this experiment and soaked the dialysis tubing in distilled water for half an hour. Once the dialysis bag had soak, I closed off one end with a rubber band and filled the dialysis bag one third the way full with Karo syrup using a funnel. I then closed off the other end of the dialysis tube with another rubber band and placed the closed dialysis bag back in a bowl filled with distilled water. Every half hour or so I would check and record my results in the table below. Next I submerged an non-cracked egg in white vinegar in a glass jar with the top sealed on. The egg sat in the vinegar for the next twenty four hours and I recorded my observations in the table below. When removing the egg, it was a soft, flexible membrane. I filled an empty glass jar half way with Karo syrup and place the soft egg in this jar with the lid sealed on. The egg again sat for twenty four hours and I
Introduction: In this lab, we are going to observe the difference in freezing point between pure water and salt water. We will also observe the permeability of an egg shell membrane and dialysis tubing, acting in place of a human cell, when placed into a hypertonic or hypotonic solution. We will observe the changes in size, shape, and characteristic.
Materials and Methods: First we begin this experiment by making an ice bath in the 100mL beaker. I filled a test tube with room temperature distilled water and recorded the temperature to be consistent at 23˚C. I put the room temperature test tube in the ice bath and recorded the change in temperature every thirty seconds. After roughly 510 seconds the temperature was consistent at 0˚C. Next I repeated the same experiment, except this time the test tube contained salt water. The temperature in the test tube dropped more quickly and consistently stayed -2˚C after just 330 seconds. I recorded my temperature readings in the table below. Next, I began part two of this experiment and soaked the dialysis tubing in distilled water for half an hour. Once the dialysis bag had soak, I closed off one end with a rubber band and filled the dialysis bag one third the way full with Karo syrup using a funnel. I then closed off the other end of the dialysis tube with another rubber band and placed the closed dialysis bag back in a bowl filled with distilled water. Every half hour or so I would check and record my results in the table below. Next I submerged an non-cracked egg in white vinegar in a glass jar with the top sealed on. The egg sat in the vinegar for the next twenty four hours and I recorded my observations in the table below. When removing the egg, it was a soft, flexible membrane. I filled an empty glass jar half way with Karo syrup and place the soft egg in this jar with the lid sealed on. The egg again sat for twenty four hours and I