Combinatorial Biotechnology
BEL718- Term Paper Mass Spectroscopy Applications in Combinatorial Biotechnology
By
Mehul Bhardwaj 2008BB50021
Table of Contents:
1. Introduction What is Mass Spectroscopy? Operating principle Steps 2. Application in Combinatorial Biotechnology Advantages/ summary 3. References
3 3 4 4 6 7 9
3
Introduction:
Mass spectrometry’s is one of the most important analytical methods, due to its outstanding characteristics such as, high sensitivity, detection limits, speed and ease of handling. Also, it can be used in various fields of science, such as analytical chemistry, pharmaceutical industry, environmental chemistry, forensic sciences etc. In analytical chemistry, it has been recently found useful in biochemical problems like proteome, metabolome, drug discovery, metabolism and so on. It has also been used to detect soil, water and food contamination and thus is a handy tool for environmental studies. Other applications include atomic physics, reaction physics, reaction kinetics, geochronology, inorganic chemical analysis, ion–molecule reactions, determination of thermodynamic parameters (Gibbs free energy, Ka, etc.), and many others.
What is Mass Spectroscopy?
A simple definition of mass spectroscopy would describe it as a method to weigh molecules by measuring the mass-to-charge ratio (m/z) of its ions. Thus, the first step in any mass spectrometer would include the ionizing if the molecules. This step should not break down the molecule or affect any other properties, but should only add a desired amount of charge (and known mass) to the molecule under investigation. The ions are then made to enter a zone, a certain mass analyzer, in which a certain electrostatic and magnetic potential exists. This zone is where the separation of molecules on the basis of m/z ratio takes place. The ions are finally detected at the end of the mass analyzer tunnel using suitable methods.
4
The ability of this technique to measure masses as small as 1 Da, is
By
Mehul Bhardwaj 2008BB50021
Table of Contents:
1. Introduction What is Mass Spectroscopy? Operating principle Steps 2. Application in Combinatorial Biotechnology Advantages/ summary 3. References
3 3 4 4 6 7 9
3
Introduction:
Mass spectrometry’s is one of the most important analytical methods, due to its outstanding characteristics such as, high sensitivity, detection limits, speed and ease of handling. Also, it can be used in various fields of science, such as analytical chemistry, pharmaceutical industry, environmental chemistry, forensic sciences etc. In analytical chemistry, it has been recently found useful in biochemical problems like proteome, metabolome, drug discovery, metabolism and so on. It has also been used to detect soil, water and food contamination and thus is a handy tool for environmental studies. Other applications include atomic physics, reaction physics, reaction kinetics, geochronology, inorganic chemical analysis, ion–molecule reactions, determination of thermodynamic parameters (Gibbs free energy, Ka, etc.), and many others.
What is Mass Spectroscopy?
A simple definition of mass spectroscopy would describe it as a method to weigh molecules by measuring the mass-to-charge ratio (m/z) of its ions. Thus, the first step in any mass spectrometer would include the ionizing if the molecules. This step should not break down the molecule or affect any other properties, but should only add a desired amount of charge (and known mass) to the molecule under investigation. The ions are then made to enter a zone, a certain mass analyzer, in which a certain electrostatic and magnetic potential exists. This zone is where the separation of molecules on the basis of m/z ratio takes place. The ions are finally detected at the end of the mass analyzer tunnel using suitable methods.
4
The ability of this technique to measure masses as small as 1 Da, is
References: Mass spectrometry and combinatorial chemistry: a short outline, Antonio T.; Maria A.; Franco C.; Alessandro S.; and Carlo M., J. Mass Spectrom. 2001; 36: 1249–1259. Applications of Mass Spectrometry in Combinatorial Chemistry, Gary S.;J. Kathleen L., BIOTECHNOLOGY AND BIOENGINEERING (COMBINATORIAL CHEMISTRY), VOL. 61, NO. 2, SPRING, 1998 Mass Spectrometry: Principles and Applications by Edmond de Hoffmann and Vincent Stroobant http://masspec.scripps.edu/mshistory/whatisms_details.php#ionization Lecture on Mass spectroscopy, BEL311, Prof. Ravi Elangovan, DBEB, IIT Delhi