Comparative Study on Regeneration Potentiality of Aromatic Indica Rice (Oryza Sativa L.) of Bangladesh

M. Ashrafuzzaman et al / Research Journal of Biology (2012), Vol. 02, Issue 03, pp. 98-103

ISSN 2049-1727

Research Paper

Comparative Study on Regeneration Potentiality of Aromatic Indica Rice (Oryza sativa L.) of Bangaldesh
Golam Gaus Mohiuddin Chowdhury1, M. Ashrafuzzaman*1, F. Raihan2 and Shamsul H. Prodhan1
1

Department of Genetic Engineering and Biotechmology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh 2 Department of Forestry and Environmental Science, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh *E-Mail: [e-mail] ; [e-mail]

Abstract
In this study, two varieties of aromatic rice (Oryza sativa L.) BRRI Dhan 50 (Bangla Moti) and BRRI Dhan 34 (Khaskhani) were used to establish a suitable system for callus initiation and regeneration. MS (Murashige & Skoog, 1962) media supplemented with different concentrations i.e. (1.0, 2.0, 3.0, 4.0 mg/L of 2,4-D (2,4-dichlorophenoxyacetic acid) and combination with BAP (6-benzylaminopurine), (2.0 mg/L) were used for callus induction from mature dehusked rice seeds. MS medium supplemented with only 3.0 mg/L of 2,4-D, produced maximum percentage of callus that is 90% for BRRI Dhan 50 and 80% for BRRI Dhan 34. On the other hand, MS media with 3.0 and 4.0 mg/L of 2,4-D in combination with 2.0 mg/L BAP produced highest percentage of callus (80 %) for BRRI Dhan 50 and 70 % for BRRI Dhan 34. For plantlet regeneration, MS media with 1.0 mg/L NAA (1-Napthaleneacetic acid), 2.0 mg/L BA (6-benzyladenine) and various concentrations of Kinetin (0.0, 1.0, 2.0, 3.0, 4.0 mg/L) were employed. The maximum percentage of shoot regeneration was recorded at MS media supplemented with 4.0 mg/L of Kinetin +1.0 mg/L NAA and 2.0 mg/L BA for both varieties. These results will be very helpful to improve rice quality through somaclonal variation and genetic transformation. Keywords: Auxin, BRRI Dhan, Cytokinin, Plantlet Regeneration,

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