Date ____________
THE EFFECT OF CONCENTRATION
ON THE RATE OF DIFFUSION PURPOSE: This experiment will explore the concentration gradient by measuring the rate of diffusion in a number of theoretical cells. The objectives for this lab are: (1) To define concentration gradient, (2) To explain experimental data concerning different concentration gradients, (3) To complete a data table and prepare a graph to illustrate the data, and (4) To apply information from this experiment to other related problems.
MATERIALS: 6 pieces of dialysis tubing Sucrose solution concentrations of 0M, .2M, .4M, .6M, .8M, 1.0M String Balance 6 beakers or plastic cups …show more content…
Tube 1) Distilled water: This bag will lose water.
b. Tube 2) .2M sucrose solution: This bag will lose water.
c. Tube 3) .4M sucrose solution: This bag is isotonic to the beaker and will remain the same in mass.
d. Tube 4) .6 M sucrose solution: This bag will gain water
e. Tube 5) .8 M sucrose solution: This bag will gain water.
f. Tube 6) 1.0 M sucrose solution: This bag will gain water. 10. A paramecium has a contractile vacuole to help pump out excess water. What will happen to the rate of contractile vacuole function if the paramecium is placed in a:
a) 100% water solution - Water will move rapidly into the Paramecium. The contractile vacuole will have to pump faster and faster to keep out excess water. If too much water enters the cell, cytolysis could occur.
b) Salt water - Water will begin to leave the cell. The contractile vacuole will stop pumping to try to conserve water.
11. A dialysis bag is filled with distilled water and then placed in a sucrose solution. The bag’s initial mass is 20 grams and its final mass is 18 grams.
a) Calculate the percent change of mass. Show all work.
b) Is the sucrose solution in the beaker isotonic, hypertonic, or hypotonic to the distilled water in the