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Cytotoxic and Genotoxic Effects of Gutkha Using Allium Cepa Assay

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Cytotoxic and Genotoxic Effects of Gutkha Using Allium Cepa Assay
Cytotoxic and Genotoxic Effects of Gutkha using Allium cepa Assay

EKTA BHATTACHARYA Roll BGC/BOT No. 112001 M.Sc Semester IV Examination 2013 Under the supervision of Dr. Shyamal Kr. Chakraborty Associate Professor, Cytogenetics Laboratory, PG Dept of Botany, Barasat Government College

Cytotoxic And Genotoxic Effects of Gutkha using Allium cepa Assay

EKTA BHATTACHARYA Roll BGC/BOT No. 112001 M.Sc Semester IV Examination 2013 Under the supervision of Dr. Shyamal Kr. Chakraborty Associate Professor, Cytogenetics Laboratory, PG Dept of Botany, Barasat Government College

CONTENTS:
List of figures …………………………………………………………………………………………………….........i
List of tables…………………………………………………………………………………………………….….…….ii
Abstract …………………………………………………………………………………………………………………...iii
List of abbreviations and acronyms …………………………………………………………………………..iv 1. Introduction and brief literature review ………………………………………………………………..1
1.1 Tobacco: a serious threat ………………………………………………………………………………1 1.2. Diseases caused ……………………………………….……………….………………………….……..1
1.3 Carcinogenic ingredients ……………………………………………………………………….………2 2. Materials and methods …………………………………………………………………………………….…3 2.1. Allium cepa chromosome assay system ………………………………………………………….3 2.2 Determination of EC50 …………………………………………………………………………………….3 2.3. Cytogenetic parameters ……………………………………………………….………………………...5 2.4. Statistical analysis ………………………………………………………………….………………………..5 3. Results ……………………………………………………………………….………………………………………….6 3.1. Macroscopic effects……………………………………………………………………………………….6 3.2. Microscopic effects ………………………………………….……………………………………………6 4. Conclusions ………………………………………………………………………….……………………….……13 5. Acknowledgements
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