Daisy Gene Knockdown Lab Report
Daisy – Gene knockdown
Purpose of genetic modification of dairy cattle
Daisy the cow, a Genetically Engineered Cloned Cow, made using SCNT cloning and RNA interference, is able to produce milk without the protein β-lactoglobulin (BLG). BLG is an allergy causing protein which around 1 in 12 infants develop and allergic response to although most infants out grown their allergy. The BLG is not found in human breast milk and it is recognised by the body as foreign and causes allergies. Currently to remove the protein from infant formula, manufacturers are breaking up the whey to remove large amounts of the protein, in an effort to decrease its reaction. By making Daisy, manufacturers can hopefully avoid breaking up the whey protein to decrease to concentration of BLG in the infant formula, and therefore lower the risk of exposure to the allergen.
Stages of SCNT Cloning SCNT Diagram
This Process was first used to Clone Dolly the Sheep in 1996, and was then used to Clone Daisy the Cow. SCNT (Somatic Cell Nuclear transfer) a technique used to clone mammal using a …show more content…
During the SCNT process, once the nucleus has been removed from the somatic cell
Explain How the process of gene knockdown effects the expression of the target gene
Explain Why this method of controlling gene expression was used
Explain How the process of SCNT cloning is achieved
Explain Why this method of cloning was used
Implications of modifying gene expression by RNA interference
1. High Caseins levels…….
2. Implication 2
Implications of SCNT Cloning
1. Genetic diversity
2. Survival of the population
Compare and Contrast two implications between the different processes
Evaluate and analyse two biological implications for either gene knockdown or SCNT cloning.
Evaluate the effectiveness of SCNT ineffective Evaluate the effectiveness of gene knockdown
Compare and
Purpose of genetic modification of dairy cattle
Daisy the cow, a Genetically Engineered Cloned Cow, made using SCNT cloning and RNA interference, is able to produce milk without the protein β-lactoglobulin (BLG). BLG is an allergy causing protein which around 1 in 12 infants develop and allergic response to although most infants out grown their allergy. The BLG is not found in human breast milk and it is recognised by the body as foreign and causes allergies. Currently to remove the protein from infant formula, manufacturers are breaking up the whey to remove large amounts of the protein, in an effort to decrease its reaction. By making Daisy, manufacturers can hopefully avoid breaking up the whey protein to decrease to concentration of BLG in the infant formula, and therefore lower the risk of exposure to the allergen.
Stages of SCNT Cloning SCNT Diagram
This Process was first used to Clone Dolly the Sheep in 1996, and was then used to Clone Daisy the Cow. SCNT (Somatic Cell Nuclear transfer) a technique used to clone mammal using a …show more content…
During the SCNT process, once the nucleus has been removed from the somatic cell
Explain How the process of gene knockdown effects the expression of the target gene
Explain Why this method of controlling gene expression was used
Explain How the process of SCNT cloning is achieved
Explain Why this method of cloning was used
Implications of modifying gene expression by RNA interference
1. High Caseins levels…….
2. Implication 2
Implications of SCNT Cloning
1. Genetic diversity
2. Survival of the population
Compare and Contrast two implications between the different processes
Evaluate and analyse two biological implications for either gene knockdown or SCNT cloning.
Evaluate the effectiveness of SCNT ineffective Evaluate the effectiveness of gene knockdown
Compare and