DAT Study Guide

Chapter 8 ASSIGNMENT - Evolution
1. The amino acid sequence for the globins (alpha, beta, delta, epsilon, gammaA, gammaG) are aligned below. The red letters in
the beta and delta sequences show the locations of replacement.
What % divergence is observed between beta and delta globin proteins? __ 13/149 = 8.7%
What % divergence is observed between alpha and delta globin proteins? 84/150= 56% ________
HUMALPHAGLOBIN HUMBETAGLOBIN HUMDELTAGLOBIN
HUMALPHAGLOBIN HUMBETAGLOBIN HUMDELTAGLOBIN
HUMALPHAGLOBIN HUMBETAGLOBIN HUMDELTAGLOBIN
1 50 MV.LSPADKT NVKAAWGKVG AHAGEYGAEA LERMFLSFPT TKTYFPHF.D MVHLTPEEKS AVTALWGKVN ..VDEVGGEA LGRLLVVYPW TQRLFESFGD MVHLTPEEKT AVNALWGKVN ..VDAVGGEA LGRLLVVYPW TQRFFESFGD
51 100 LS.....HGS AQVKGHGKKV ADALTNAVAH VDDMPNALSA LSDLHAHKLR LFTPDAVMGN PKVKAHGKKV LGAFSDGPAH LDNLKGTFAT LSELHCDKLH LSSPDAVMGN PKVKAHGKKV LGAFSDGLAH LDNLKGTFSQ LSELHCDKLH
101 150 VDPVNFKLLS HCLLVTLAAH LPAEFTPAVH ASLDKFLASV STVLTSKYR. VDPENFRLLG NVLVCVLAHH FGKEFTPPVQ AAYQKVVAGV ANALAHKYH. VDPENFRLLG NVLVCVLARN FGKEFTPQMQ AAYQKVVAGV ANALAHKYH.
The nucleotide sequences for the same genes is aligned below. The red letters represent non-synonymous
replacements and the green letters are silent synonymous substitutions.
HUMALPHAGLOBIN CDSHUMALPHAGLOBIN CDSHUMBETAGLOBIN CDSHUMDELTAGLOBIN HUMDELTAGLOBIN
HUMALPHAGLOBIN CDSHUMALPHAGLOBIN CDSHUMBETAGLOBIN CDSHUMDELTAGLOBIN HUMDELTAGLOBIN
HUMALPHAGLOBIN CDSHUMALPHAGLOBIN CDSHUMBETAGLOBIN CDSHUMDELTAGLOBIN HUMDELTAGLOBIN
HUMALPHAGLOBIN CDSHUMALPHAGLOBIN CDSHUMBETAGLOBIN CDSHUMDELTAGLOBIN HUMDELTAGLOBIN
HUMALPHAGLOBIN CDSHUMALPHAGLOBIN CDSHUMBETAGLOBIN CDSHUMDELTAGLOBIN HUMDELTAGLOBIN
HUMALPHAGLOBIN CDSHUMALPHAGLOBIN CDSHUMBETAGLOBIN
1 51 MV.LSPADKTNVKAAWG ATG GTG ... CTG TCT CCT GCC GAC AAG ACC AAC GTC AAG GCC GCC TGG GGT ATG GTG CAC CTG ACT CCT GAG GAG AAG TCT GCC GTT ACT GCC CTG TGG GGC ATG GTG CAT CTG ACT CCT GAG GAG AAG ACT GCT GTC AAT GCC CTG TGG GGC MVHLTPEEKTAVNALWG
KVGAHAGEYGAEALERM AAG GTC GGC GCG CAC GCT GGC